Cancer Research Technology
Log in Register
Menu

Anti-Haemoglobin Scavenger Receptor [GHI/61]

Invented by Karen Pulford at University of Oxford

Info

Catalogue Number 151380
Applications FACS IHC IF IP Fn WB
Antigen/Gene or Protein Targets Haemoglobin Scavenger Receptor (M130; CD163)
Reactivity Human
Relevance Monoclonal antibody which recognises circulating blood monocytes and the large majority of tissue macrophages, without labelling granulocytes or lymphocytes.

Background and Research Application
Anti-haemoglobin scavenger receptor labels the majority of monocytes and tissue macrophages. It recognises a membrane glycoprotein with molecular weight 130,000 (reduced) and 110,000 (unreduced). The glycoprotein antigen is expressed exclusively on the cell surface of human monocytes and macrophages, which evolves predominantly in the late phase of inflammation. The glycoprotein GHI/61 binds to CD163, a transmembrane glycoprotein belonging to the scavenger receptor cysteine-rich superfamily. It is a receptor for the haemoglobin-haptoglobin complex.
CD163 expression on monocytes and macrophages changes as these cells differentiate, as such GHI/61 can detect levels of differentiated monocytes and macrophages. CD163 has high affinity for the haemoglobin-haptoglobin complex and upon ligand binding, leads to the production of IL-6 and IL-10.

GHI/61 monoclonal antibody was created to label monocytes and macrophage with increased specificity, given similar antibodies frequently label lymphocytes etc., or only recognise a particular subset of macrophages.
Host Mouse
Immunogen Hairy cell leukaemia cells
Immunogen UniProt ID Q86VB7
Subclass IgG1
Myeloma Used P3/NS1/1-Ag4.1
Recommended Growing Conditions RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT
Notes Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Points of Interest
GHI/61 does not label lymphocytes, which is a drawback of other monocyte labelling antibodies. GHI/61 only reacted with normal monocytes and macrophages when tested upon a variety of leukaemia’s and lymphomas. All monocytes were positive for FACS staining following permeabilization, with no lymphocytes or granulocytes stained, giving more accurate results. The absence of granulocyte (and its precursors) staining may be of value within leukaemia and lymphoma diagnosis.

GHI/61 antibody recognises the same antigen as Ber-MAC3, Ki-M8 and SM4. However, it seems all four label different epitopes of the same antigen.
Labelling of the tonsil indicated GHI/61 didn’t recognise interdigitating reticulum cells, dendritic reticulum cells or Langerhans cells.

Ultroser G can be used at 1% if the cells are not growing well.

Concentration
1mg/ml as standard
Research Area Cardiovascular, Immunology

References

There are 15 reference entries for this reagent.

View All References

References: 15 entries

Salerno-Goncalves et al. 2019. PLoS Negl Trop Dis. 13(8):e0007650. PMID: 31412039.

Amini et al. 2019. BMC Cancer. 19(1):316. PMID: 30953461.

Schulz et al. 2019. Sci Rep. 9(1):1925. PMID: 30760760.

Silva et al. 2019. Front Immunol. 10:1508. PMID: 31333653.

Gruenbacher et al. 2019. Front Immunol. 10:1870. PMID: 31447857.

Moura et al. 2012. Eur J Immunol. 42(11):2925-36. PMID: 22851198.

CD163 favors Mycobacterium leprae survival and persistence by promoting anti-inflammatory pathways in lepromatous macrophages.

Europe PMC ID: 22851198

Moniuszko et al. 2006. Clin Vaccine Immunol. 13(6):704-7. PMID: 16760331.

Monocyte CD163 and CD36 expression in human whole blood and isolated mononuclear cell samples: influence of different anticoagulants.

Europe PMC ID: 16760331

Møller et al. 2002. Blood. 99(1):378-80. PMID: 11756196.

Identification of the hemoglobin scavenger receptor/CD163 as a natural soluble protein in plasma.

Europe PMC ID: 11756196

Law et al. 1993. Eur J Immunol. 23(9):2320-5. PMID: 8370408.

A new macrophage differentiation antigen which is a member of the scavenger receptor superfamily.

Europe PMC ID: 8370408

Pulford et al. 1992. Immunology. 75(4):588-95. PMID: 1592433.

A monocyte/macrophage antigen recognized by the four antibodies GHI/61, Ber-MAC3, Ki-M8 and SM4.

Europe PMC ID: 1592433


Add a reference

References: 15 entries

Salerno-Goncalves et al. 2019. PLoS Negl Trop Dis. 13(8):e0007650. PMID: 31412039.

Amini et al. 2019. BMC Cancer. 19(1):316. PMID: 30953461.

Schulz et al. 2019. Sci Rep. 9(1):1925. PMID: 30760760.

Silva et al. 2019. Front Immunol. 10:1508. PMID: 31333653.

Gruenbacher et al. 2019. Front Immunol. 10:1870. PMID: 31447857.

Moura et al. 2012. Eur J Immunol. 42(11):2925-36. PMID: 22851198.

CD163 favors Mycobacterium leprae survival and persistence by promoting anti-inflammatory pathways in lepromatous macrophages.

Moniuszko et al. 2006. Clin Vaccine Immunol. 13(6):704-7. PMID: 16760331.

Monocyte CD163 and CD36 expression in human whole blood and isolated mononuclear cell samples: influence of different anticoagulants.

Møller et al. 2002. Blood. 99(1):378-80. PMID: 11756196.

Identification of the hemoglobin scavenger receptor/CD163 as a natural soluble protein in plasma.

Law et al. 1993. Eur J Immunol. 23(9):2320-5. PMID: 8370408.

A new macrophage differentiation antigen which is a member of the scavenger receptor superfamily.

Pulford et al. 1992. Immunology. 75(4):588-95. PMID: 1592433.

A monocyte/macrophage antigen recognized by the four antibodies GHI/61, Ber-MAC3, Ki-M8 and SM4.


Add a reference