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Anti-Integrin b3 [Y2/51]
RECENTLY UPDATED

Invented by Dr Jacqueline Cordell at University of Oxford

Info

Catalogue Number 151367
Applications FACS IHC IF WB
Antigen/Gene or Protein Targets Integrin ß3 (CD61; GPIIIa)
Synonyms Integrin Subunit Beta; Integrin; Beta 3 (Platelet Glycoprotein IIIa; Antigen CD61); Platelet Membrane Glycoprotein IIIa; Antigen CD61; GPIIIa; GP3A; Platelet Glycoprotein IIIa; Integrin Beta 3; CD61 Antigen; BDPLT16; BDPLT2; CD61; GT
Reactivity Human
Relevance
RECENTLY UPDATED
Monoclonal antibody which can detect CD61 marker on megakaryocytes.

Background and Research Application
Integrins are heterodimeric cell surface receptors composed of alpha and beta subunits, which mediate cell-cell and cell-extracellular matrix attachments. Changes in integrin expression have been shown to be important for the growth and early metastatic capacity of melanoma cells and aberrant integrin expression has been found in many epithelial tumours.
CD61/ integrin b3 is a glycoprotein found on megakaryocytes, osteoclasts, platelets and their precursors. CD61 antigen plays a role in platelet aggregation and also as a receptor for fibrinogen, fibronectin, von Willebrand factor and vitronectrin.
This antibody is widely used as a marker of megakaryocytes in bone marrow trephines.
Host Mouse
Immunogen Phytohaemagglutin-stimulated peripheral blood cells
Immunogen UniProt ID
RECENTLY UPDATED
P05106
Subclass IgG1
Myeloma Used P3/NS1/1-Ag4.1
Recommended Growing Conditions RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT
Notes
RECENTLY UPDATED
Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Points of Interest
Diseases associated with CD61 dysfunction include Glanzmann Thrombasthenia and Platelet type-16 Bleeding Disorder.

Ultroser G can be used at 1% if the cells are not growing well.

Concentration
1mg/ml as standard
Research Area Adhesion, Cardiovascular, Cell Signaling & Signal Transduction

References

There are 15 reference entries for this reagent.

View All References

References: 15 entries
RECENTLY UPDATED

Gravastrand et al. 2019. J Immunol. 203(4):853-863. PMID: 31270150.

Charnigo et al. 2019. Clin Transl Sci. 12(2):180-188. PMID: 30597771.

Berckmans et al. 2019. J Extracell Vesicles. 8(1):1688936. PMID: 31762964.

Silasi et al. 2019. Blood Adv. 3(4):658-669. PMID: 30808684.

Blair et al. 2018. Sci Rep. 8(1):10300. PMID: 29985398.

Kahr et al. 2017. Nat Commun. 8:14816. PMID: 28368018.

Del Sordo et al. 2017. Clin Nephrol Case Stud. 5:70-77. PMID: 29350220.

Brune et al. 2007. Eur Cell Mater. 14:78-90; discussion 90-1. PMID: 18085506.

In vitro comparison of human fibroblasts from intact and ruptured ACL for use in tissue engineering.

Europe PMC ID: 18085506

Gatter et al. 1988. Histopathology. 13(3):257-67. PMID: 3192191.

The immunohistological detection of platelets, megakaryocytes and thrombi in routinely processed specimens.

Europe PMC ID: 3192191

Vinci et al. 1984. Br J Haematol. 56(4):589-605. PMID: 6231944.

Immunological study of in vitro maturation of human megakaryocytes.

Europe PMC ID: 6231944

Nachman et al. 1982. J Clin Invest. 69(2):263-9. PMID: 6460044.

Complex formation of platelet membrane glycoproteins IIb and IIIa with fibrinogen.

Europe PMC ID: 6460044


Add a reference

References: 15 entries
RECENTLY UPDATED

Gravastrand et al. 2019. J Immunol. 203(4):853-863. PMID: 31270150.

Charnigo et al. 2019. Clin Transl Sci. 12(2):180-188. PMID: 30597771.

Berckmans et al. 2019. J Extracell Vesicles. 8(1):1688936. PMID: 31762964.

Silasi et al. 2019. Blood Adv. 3(4):658-669. PMID: 30808684.

Blair et al. 2018. Sci Rep. 8(1):10300. PMID: 29985398.

Kahr et al. 2017. Nat Commun. 8:14816. PMID: 28368018.

Del Sordo et al. 2017. Clin Nephrol Case Stud. 5:70-77. PMID: 29350220.

Brune et al. 2007. Eur Cell Mater. 14:78-90; discussion 90-1. PMID: 18085506.

In vitro comparison of human fibroblasts from intact and ruptured ACL for use in tissue engineering.

Gatter et al. 1988. Histopathology. 13(3):257-67. PMID: 3192191.

The immunohistological detection of platelets, megakaryocytes and thrombi in routinely processed specimens.

Vinci et al. 1984. Br J Haematol. 56(4):589-605. PMID: 6231944.

Immunological study of in vitro maturation of human megakaryocytes.

Nachman et al. 1982. J Clin Invest. 69(2):263-9. PMID: 6460044.

Complex formation of platelet membrane glycoproteins IIb and IIIa with fibrinogen.


Add a reference