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Anti-CD45RB [PD7/26]

Invented by Dr Jacqueline Cordell at University of Oxford

Info

Catalogue Number 151364
Applications FACS IHC IF WB
Antigen/Gene or Protein Targets Protein tyrosine phosphatase, receptor type, C (PTPRC, CD45RB)
Synonyms Protein Tyrosine Phosphatase; Receptor Type C; CD45 Antigen; CD45; L-CA; T200; Protein Tyrosine Phosphatase; Receptor Type; C Polypeptide; T200 Leukocyte Common Antigen; T200 Glycoprotein; EC 3.1.3.48; GP180; CD45R; B220; LCA; LY5
Reactivity Human
Relevance Monoclonal antibody capable of detecting CD45RB in paraffin-embedded samples, and aids in differentiating between lymphoid and non-lymphoid tumours of B-cell origin.

Background and Research Application
CD45RB is a protein tyrosine phosphatase receptor that is present on most hematopoietic cells and absent on non-hematopoietic cells. It is a transmembrane pan-leukocyte protein with tyrosine phosphatase activity and is involved in the regulation of signal transduction in haematopoiesis and the threshold of T cell antigen receptor (TCR) signalling. This is via dephosphorylation of protein tyrosine kinases (e.g. Lck and Fyn).
Expression of CD45RB is more limited than CD45 and can only be found on T cell subsets, B, NK, and myeloid cells. Regulatory T cells (Tregs) have been shown to express low levels of CD45RB, linked to increased migration to sites of infection. CD45RB is expressed on the majority of lymphomas and leukaemia’s of B-cell origin. Anti-CD45RB is useful in differentiating between lymphoid and non-lymphoid tumours.
CD45 regulates Src-family kinases, integrin-mediated signal transduction pathways and can suppress JAK kinases, acting as a negative regulator of cytokine receptor signalling. All CD45 isoforms share the same transmembrane and cytoplasmic domains, however their extracellular domains vary, depending on the spliced product.
Anti-CD45RB is capable of recognising antigens in fixed and paraffin-embedded tissues, unlike other reagents of similar specificity. This antibody reacts with an epitope which does not become denatured or masked during fixation and embedding.
Host Mouse
Immunogen Human PBL maintained in T-cell growth factor
Immunogen UniProt ID P08575
Subclass IgG1
Myeloma Used P3/NS1/1-Ag4.1
Recommended Growing Conditions RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT
Notes Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Points of Interest
There are multiple CD45R isoforms resulting from the alternative splicing of this gene, with PD7/26 antibody binding to the exon 5. High CD45RB expression results in IL-2 and IFN gamma production instead of IL-4 in vitro.

Ultroser G can be used at 1% if the cells are not growing well.

Concentration
1mg/ml as standard
Research Area Cancer, Cell Type or Organelle Marker, Cell Signaling & Signal Transduction, Immunology, Stem Cell Biology

References

There are 16 reference entries for this reagent.

View All References

References: 16 entries

Tomita et al. 2015. J Clin Exp Hematop. 55(1):45-9. PMID: 26106007.

IHC

Preza et al. 2015. PLoS One. 10(4):e0122723. PMID: 25856343.

Lapa et al. 2015. PLoS One. 10(3):e0122269. PMID: 25807228.

Chijiwa et al. 2015. Int J Oncol. :. PMID: 25963555.

IHC

Tsuneki et al. 2015. Lab Invest. :. PMID: 25961170.

IHC

A hydrogel-endothelial cell implant mimics infantile hemangioma: modulation by survivin and the Hippo pathway.

Europe PMC ID: 25961170

Establishment of patient-derived cancer xenografts in immunodeficient NOG mice.

Europe PMC ID: 25963555

Brand et al. 2015. J Biol Chem. :. PMID: 25918170.

IF

Transforming Growth Factor-β and Interleukin-1β Signaling Pathways Converge on the Chemokine CCL20 Promoter.

Europe PMC ID: 25918170

T lymphocyte density and distribution in human colorectal mucosa, and inefficiency of current cell isolation protocols.

Europe PMC ID: 25856343

Tumor-associated macrophages in glioblastoma multiforme-a suitable target for somatostatin receptor-based imaging and therapy?

Europe PMC ID: 25807228

Histiocytic Sarcoma Originating in the Lung in a 16-Year-Old Male.

Europe PMC ID: 26106007

Warnke et al. 1983. N Engl J Med. 309(21):1275-81. PMID: 6355845.

Diagnosis of human lymphoma with monoclonal antileukocyte antibodies.

Europe PMC ID: 6355845

Gatter et al. 1983. J Biol Response Mod. 2(4):369-95. PMID: 6196454.

Monoclonal antibodies in diagnostic pathology: techniques and applications.

Europe PMC ID: 6196454


Add a reference

References: 16 entries

Tomita et al. 2015. J Clin Exp Hematop. 55(1):45-9. PMID: 26106007.

IHC

Preza et al. 2015. PLoS One. 10(4):e0122723. PMID: 25856343.

Lapa et al. 2015. PLoS One. 10(3):e0122269. PMID: 25807228.

Chijiwa et al. 2015. Int J Oncol. :. PMID: 25963555.

IHC

Tsuneki et al. 2015. Lab Invest. :. PMID: 25961170.

IHC

A hydrogel-endothelial cell implant mimics infantile hemangioma: modulation by survivin and the Hippo pathway.

Establishment of patient-derived cancer xenografts in immunodeficient NOG mice.

Brand et al. 2015. J Biol Chem. :. PMID: 25918170.

IF

Transforming Growth Factor-β and Interleukin-1β Signaling Pathways Converge on the Chemokine CCL20 Promoter.

T lymphocyte density and distribution in human colorectal mucosa, and inefficiency of current cell isolation protocols.

Tumor-associated macrophages in glioblastoma multiforme-a suitable target for somatostatin receptor-based imaging and therapy?

Histiocytic Sarcoma Originating in the Lung in a 16-Year-Old Male.

Warnke et al. 1983. N Engl J Med. 309(21):1275-81. PMID: 6355845.

Diagnosis of human lymphoma with monoclonal antileukocyte antibodies.

Gatter et al. 1983. J Biol Response Mod. 2(4):369-95. PMID: 6196454.

Monoclonal antibodies in diagnostic pathology: techniques and applications.


Add a reference