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Anti-CD45RB [PD7/26]

Invented by Dr Jacqueline Cordell at University of Oxford

Info

Catalogue Number 151364
Applications FACS IHC IF WB
Antigen/Gene or Protein Targets Protein tyrosine phosphatase, receptor type, C (PTPRC, CD45RB)
Synonyms Protein Tyrosine Phosphatase; Receptor Type C; CD45 Antigen; CD45; L-CA; T200; Protein Tyrosine Phosphatase; Receptor Type; C Polypeptide; T200 Leukocyte Common Antigen; T200 Glycoprotein; EC 3.1.3.48; GP180; CD45R; B220; LCA; LY5
Reactivity Human
Relevance PTPRC (CD45) is a transmembrane tyrosine phosphatase that is present on all leukocytes. PTPRC regulates the threshold of T cell antigen receptor (TCR) signaling through dephosphorylation of protein tyrosine kinases (e.g. Lck and Fyn). CD45RB is an isoform of the PTPRC (CD45) protein which is expressed on peripheral B cells, T cytotoxic cells and CD4+ T cells. It has a role in cell signalling regulation through the activation of the Src family of tyrosine kinases. PD7/26 is widely used, in conjunction the CD45 antibody 2B11, to detect white cell-derived tumours in paraffin sections.
Host Mouse
Immunogen Human PBL maintained in T-cell growth factor
Subclass IgG1
Myeloma Used P3/NS1/1-Ag4.1
Recommended Growing Conditions RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT
Notes Ultroser G can be used at 1% if the cells are not growing well.
Research Area Cancer, Cell Type or Organelle Marker, Cell Signaling & Signal Transduction, Immunology, Stem Cell Biology

References: 16 entries

Tomita et al. 2015. J Clin Exp Hematop. 55(1):45-9. PMID: 26106007.

IHC

Preza et al. 2015. PLoS One. 10(4):e0122723. PMID: 25856343.

Lapa et al. 2015. PLoS One. 10(3):e0122269. PMID: 25807228.

Chijiwa et al. 2015. Int J Oncol. :. PMID: 25963555.

IHC

Tsuneki et al. 2015. Lab Invest. :. PMID: 25961170.

IHC

A hydrogel-endothelial cell implant mimics infantile hemangioma: modulation by survivin and the Hippo pathway.

Europe PMC ID: 25961170

Establishment of patient-derived cancer xenografts in immunodeficient NOG mice.

Europe PMC ID: 25963555

Brand et al. 2015. J Biol Chem. :. PMID: 25918170.

IF

Transforming Growth Factor-β and Interleukin-1β Signaling Pathways Converge on the Chemokine CCL20 Promoter.

Europe PMC ID: 25918170

T lymphocyte density and distribution in human colorectal mucosa, and inefficiency of current cell isolation protocols.

Europe PMC ID: 25856343

Tumor-associated macrophages in glioblastoma multiforme-a suitable target for somatostatin receptor-based imaging and therapy?

Europe PMC ID: 25807228

Histiocytic Sarcoma Originating in the Lung in a 16-Year-Old Male.

Europe PMC ID: 26106007

Warnke et al. 1983. N Engl J Med. 309(21):1275-81. PMID: 6355845.

Diagnosis of human lymphoma with monoclonal antileukocyte antibodies.

Europe PMC ID: 6355845

Gatter et al. 1983. J Biol Response Mod. 2(4):369-95. PMID: 6196454.

Monoclonal antibodies in diagnostic pathology: techniques and applications.

Europe PMC ID: 6196454


Add a reference

References: 16 entries

Tomita et al. 2015. J Clin Exp Hematop. 55(1):45-9. PMID: 26106007.

IHC

Preza et al. 2015. PLoS One. 10(4):e0122723. PMID: 25856343.

Lapa et al. 2015. PLoS One. 10(3):e0122269. PMID: 25807228.

Chijiwa et al. 2015. Int J Oncol. :. PMID: 25963555.

IHC

Tsuneki et al. 2015. Lab Invest. :. PMID: 25961170.

IHC

A hydrogel-endothelial cell implant mimics infantile hemangioma: modulation by survivin and the Hippo pathway.

Europe PMC ID: 25961170

Establishment of patient-derived cancer xenografts in immunodeficient NOG mice.

Europe PMC ID: 25963555

Brand et al. 2015. J Biol Chem. :. PMID: 25918170.

IF

Transforming Growth Factor-β and Interleukin-1β Signaling Pathways Converge on the Chemokine CCL20 Promoter.

Europe PMC ID: 25918170

T lymphocyte density and distribution in human colorectal mucosa, and inefficiency of current cell isolation protocols.

Europe PMC ID: 25856343

Tumor-associated macrophages in glioblastoma multiforme-a suitable target for somatostatin receptor-based imaging and therapy?

Europe PMC ID: 25807228

Histiocytic Sarcoma Originating in the Lung in a 16-Year-Old Male.

Europe PMC ID: 26106007

Warnke et al. 1983. N Engl J Med. 309(21):1275-81. PMID: 6355845.

Diagnosis of human lymphoma with monoclonal antileukocyte antibodies.

Europe PMC ID: 6355845

Gatter et al. 1983. J Biol Response Mod. 2(4):369-95. PMID: 6196454.

Monoclonal antibodies in diagnostic pathology: techniques and applications.

Europe PMC ID: 6196454


Add a reference

References: 16 entries

Tomita et al. 2015. J Clin Exp Hematop. 55(1):45-9. PMID: 26106007.

IHC

Preza et al. 2015. PLoS One. 10(4):e0122723. PMID: 25856343.

Lapa et al. 2015. PLoS One. 10(3):e0122269. PMID: 25807228.

Chijiwa et al. 2015. Int J Oncol. :. PMID: 25963555.

IHC

Tsuneki et al. 2015. Lab Invest. :. PMID: 25961170.

IHC

A hydrogel-endothelial cell implant mimics infantile hemangioma: modulation by survivin and the Hippo pathway.

Establishment of patient-derived cancer xenografts in immunodeficient NOG mice.

Brand et al. 2015. J Biol Chem. :. PMID: 25918170.

IF

Transforming Growth Factor-β and Interleukin-1β Signaling Pathways Converge on the Chemokine CCL20 Promoter.

T lymphocyte density and distribution in human colorectal mucosa, and inefficiency of current cell isolation protocols.

Tumor-associated macrophages in glioblastoma multiforme-a suitable target for somatostatin receptor-based imaging and therapy?

Histiocytic Sarcoma Originating in the Lung in a 16-Year-Old Male.

Warnke et al. 1983. N Engl J Med. 309(21):1275-81. PMID: 6355845.

Diagnosis of human lymphoma with monoclonal antileukocyte antibodies.

Gatter et al. 1983. J Biol Response Mod. 2(4):369-95. PMID: 6196454.

Monoclonal antibodies in diagnostic pathology: techniques and applications.


Add a reference


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