Anti-CR2 [21A/5]
Invented by Dr Jacqueline Cordell from University of Oxford
Invented at University of Oxford
- Datasheet
- References (1)
- Inventor Info
Info
| Catalogue Number | 151354 |
| Applications | FACS IHC |
| Antigen/Gene or Protein Targets | Complement component (3d/Epstein Barr virus) receptor 2 (CR2, CD21) |
| Synonyms | Complement Component 3d Receptor 2; Epstein-Barr Virus Receptor 2; Complement C3d Receptor; EBV Receptor; C3DR; CD21 Antigen; CVID7; SLEB9; CD21; Cr2; CR |
| Reactivity | Human |
| Relevance |
Monoclonal antibody directed against CR2, involved in allergic responses and inflammatory diseases. Background and Research Application CR2 is expressed strongly on mature B cells, follicular dendritic cells and weakly on immature thymocytes and T lymphocytes. In B-cell ontogeny, CR2 appears after the pre-B-stage, is maintained during peripheral B-cell development and is lost upon terminal differentiation into plasma cells. CR2 expression is also gradually lost after stimulation of B cells in vitro. CR2 functions as a receptor for C3d, C3dg and iC3b Complement components and for EBV and for IFN alpha. CR2 binds to CD23 and associates with CD19, CD81 and Leu13 to form a large signal-transduction complex involved in B-cell activation. By inducing release of proinflammatory cytokines and upregulating expression of molecules involved in antigen presentation, CR2 modulates critical monocyte functions that may be relevant to allergic and inflammatory disorders. |
| Host | Mouse |
| Immunogen | CD21 |
| Immunogen UniProt ID | P20023 |
| Subclass | IgG1 kappa |
| Myeloma Used | P3/NS1/1-Ag4.1 |
| Recommended Growing Conditions | RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT |
| Notes |
Production Details Purified using multi-step affinity chromatography with protein A. Storage Conditions Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles. Points of Interest Genetic variations in the CD21 gene are associated with susceptibility to systemic lupus erythematosus type 9 (SLEB9). Alternatively, spliced transcript variants encoding different isoforms of CD21 have been found. Ultroser G can be used at 1% if the cells are not growing well. Concentration 1mg/ml as standard |
| Research Area | Cell Signaling & Signal Transduction, Immunology |
References: 1 entry
CD Guide for CD21 (1989) In Knapp W, et al (eds) Leucocyte Typing IV, Oxford University Press, Oxford, New York and Tokyo, p 1080. ISBN-13: 978-0192618672
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References: 1 entry
CD Guide for CD21 (1989) In Knapp W, et al (eds) Leucocyte Typing IV, Oxford University Press, Oxford, New York and Tokyo, p 1080. ISBN-13: 978-0192618672
Add a reference
