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Anti-CALLA [SS2/36]


Catalogue Number 151348
Applications FACS IHC WB
Antigen/Gene or Protein Targets Common Acute Lymphocytic Leukaemia Antigen (CALLA, CD10)
Reactivity Human
Relevance CALLA is expressed on B- and T- cell precursors, bone marrow stromal cells, lymphoblastic, Burkitt’s, and follicular germinal center lymphomas, and on cells from patients with chronic myelocytic leukemia (CML). CALLA is a cell surface enzyme that inactivates a variety of peptides. SS2/36 is a marker for Acute Lymphocytic Luekaemia (ALL). CALLA is widely used for identification of "common" type Acute Lymphocytic Leukaemia (ALL).
Host Mouse
Immunogen Common acute lymphoblastic leukaemia cells
Subclass IgG1
Myeloma Used P3/NS1/1-Ag4.1
Recommended Growing Conditions RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT
Notes Ultroser G can be used at 1% if the cells are not growing well.
Research Area Cancer, Cell Type or Organelle Marker, Immunology, Stem Cell Biology

References: 1 entry

Majdic O, Sugita K, Stockinger H, Skrobal A, Knapp W (1987) Comparative evaluation of CD10 antibodies. In McMichael AJ, et al (eds) Leucocyte Typing III, Oxford University Press, Oxford, New York and Tokyo, p 488-9.

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