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Anti-Rad51B [Rad51B 1E11/6]


Catalogue Number 151281
Applications WB
Antigen/Gene or Protein Targets Rad51B
Reactivity Human and Hamster
Relevance RAD51B is a Rad51 paralog. RAD51 is a eukaryotic homologue of E. coli RecA, a recombinase, and a component of the homologous recombination DNA repair pathway. RAD51 forms a nucleoprotein filament (through binding RAD52 and single stranded DNA that are exposed following double strand breaks) that initiates recombination. RAD51B is also a component for the homologous recombination pathway.
Host Mouse
Immunogen Raised against His-tagged human Rad51B, overexpressed in e.coli and purified on a talon affinity column under denaturing conditions, followed by gel purification using SDS-PAGE.
Subclass IgG1
Myeloma Used Sp2/0-Ag14
Recommended Growing Conditions DMEM + 5% FCS
Strain Balb/c
Notes Detects as little as 10ng of purified protein. Recommended for detection of Rad51B of human and hamster origin by Western Blotting (starting dilution 1:200, dilution range 1:100-1:1000) and immunoprecipitation [1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)].
Research Area DNA Damage and Repair, Epigenetics & Nuclear Signalling

References: 1 entry

French et al. 2002. J Biol Chem. 277(22):19322-30. PMID: 11912211.

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