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Monoclonal Antibodies

Anti-SGII [SGII 9G3/3]

Invented by Dr Sharon Tooze from The Francis Crick Institute
Invented at Cancer Research UK London Research Institute: Lincoln's Inn Fields
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Adapted from Røsjø et al. 2012. PLoS One. 7(5):e37401. PMID: 22655045. Figure. SgII is produced by cardiomyocytes and increased in the left ventricle during HF development. B, Representative photomicrographs of myocardial tissue sections of a HF mouse demonstrating SgII immunoreactivity (brown staining) in cardiomyocytes of the non-infarcted LV (upper left image). Images of the area bordering the infarcted zone (border zone) are presented in the upper right image (magnification: ×100) and the lower left image (magnification: ×400) and demonstrate SgII immunoreactivity also in non-cardiomyocyte cardiac cells, including fibroblasts. In the upper right image, the infarct area is seen on the left side with granulation tissue in between non-infarcted tissue in the center. Bottom right picture demonstrates very weak staining after use of non-immune rabbit serum as control (ctr). Magnification: ×100 except lower left image (×400).
Adapted from Uys JD, Grey AC, Wiggins A, Schwacke JH, Schey KL, Kalivas PW. Matrix-assisted laser desorption/ionization tissue profiling of secretoneurin in the nucleus accumbens shell from cocaine-sensitized rats. J Mass Spectrom. 2010 Jan;45(1):97-103. doi: 10.1002/jms.1697. PMID: 19918966; PMCID: PMC4153729. Western blot analysis of secretogranin II. (A) Secretogranin II was upregulated following 3 weeks of abstinence after cocaine sensitization. Data points are depicted as mean ± SEM fold change in optical density from saline (S) and cocaine (C) animals. N = 8/group. * p ≤ 0.05 comparing S with C using a Student’s t-test. Data were normalized using GAPDH as loading control. (B) Representative secretogranin II and GADPH immunoblot.
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Catalogue Number 151208
Applications FACS IHC IP WB
Antigen/Gene or Protein Targets Secretogranin (SG II)
Reactivity Mouse and Rat
Relevance SgII is a member of the chromogranin family of secretory proteins. SgII is stored in neuroendocrine cells involved in hormone packaging. SGII 9G3/3 has potential uses for chromatogranin diagnostics.
Host Mouse
Subclass IgG2b
Myeloma Used Sp2/0-Ag14
Recommended Growing Conditions DMEM + 5% FCS
Research Area Neurobiology

References

There are 8 reference entries for this reagent.

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References: 8 entries

Røsjø et al. 2012. PLoS One. 7(5):e37401. PMID: 22655045.

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Secretogranin II; a protein increased in the myocardium and circulation in heart failure with cardioprotective properties.

Europe PMC ID: 22655045

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Uys et al. 2010. J Mass Spectrom. 45(1):97-103. PMID: 19918966.

View  

Matrix-assisted laser desorption/ionization tissue profiling of secretoneurin in the nucleus accumbens shell from cocaine-sensitized rats.

Europe PMC ID: 19918966

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Urbé et al. 1998. J Cell Biol. 143(7):1831-44. PMID: 9864358.

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Homotypic fusion of immature secretory granules during maturation in a cell-free assay.

Europe PMC ID: 9864358

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Dittié et al. 1995. Biochem J. 310 ( Pt 3):777-87. PMID: 7575410.

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Characterization of the endopeptidase PC2 activity towards secretogranin II in stably transfected PC12 cells.

Europe PMC ID: 7575410

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References: 8 entries

Røsjø et al. 2012. PLoS One. 7(5):e37401. PMID: 22655045.

View  

Secretogranin II; a protein increased in the myocardium and circulation in heart failure with cardioprotective properties.

View  

Uys et al. 2010. J Mass Spectrom. 45(1):97-103. PMID: 19918966.

View  

Matrix-assisted laser desorption/ionization tissue profiling of secretoneurin in the nucleus accumbens shell from cocaine-sensitized rats.

View  

Urbé et al. 1998. J Cell Biol. 143(7):1831-44. PMID: 9864358.

View  

Homotypic fusion of immature secretory granules during maturation in a cell-free assay.

View  

Dittié et al. 1995. Biochem J. 310 ( Pt 3):777-87. PMID: 7575410.

View  

Characterization of the endopeptidase PC2 activity towards secretogranin II in stably transfected PC12 cells.

View  

Add a reference

Inventor Information