Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
0.25-0.5 μg/ml |
0.5-1 μg/ml |
Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
chicken fibroblast, FS11
Product Note
Recognizes an epitope located at the C-terminal end of the α-tubulin isoform in a variety of organisms.
Form
Liquid
Buffer
PBS
Preservative
15mM Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
~2? mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Sea Urchin
Immunogen
Sarkosyl-resistant filaments from Strongylocentrotus purpuratus (sea urchin) sperm axonemes.
Purification
Purified immunoglobulin
Conjugation
Unconjugated
RRID
AB_373242
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Synonyms
tubulin alpha 4a , ALS22 , H2-ALPHA , TUBA1
Cellular Localization
Cytoplasm, cytoskeleton
Background
Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. Tubulin is a heterodimer which consists of alpha tubulin and beta tubulin.
Database
Research Area