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TIMP2 antibody [3A4]

Orthogonal
Anti-TIMP2 antibody [3A4] used in IHC (Paraffin sections) (IHC-P). GTX21828
Anti-TIMP2 antibody [3A4] used in Western Blot (WB). GTX21828

Cat. No. GTX21828

Reference 4

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3A4

Isotype

 IgG2a

Application

 WB, IHC-P

Reactivity

 Human
Package
250 μl ($319)
See all TIMP2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-P 1:25‐1:50

Note :

IHC-P
Recommendations for antigen retrieval : Citrate Buffer pH 6.0 at room temperature for 30 mins.
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

BALB/C mice were injected with a synthetic peptide from the N-terminal region of human TIMP-2.

Purification

Purified IgG

Conjugation

Unconjugated

RRID

AB_372322

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

TIMP metallopeptidase inhibitor 2 , CSC-21K , DDC8

Cellular Localization

Secreted

Background

This gene is a member of the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. In addition to an inhibitory role against metalloproteinases, the encoded protein has a unique role among TIMP family members in its ability to directly suppress the proliferation of endothelial cells. As a result, the encoded protein may be critical to the maintenance of tissue homeostasis by suppressing the proliferation of quiescent tissues in response to angiogenic factors, and by inhibiting protease activity in tissues undergoing remodelling of the extracellular matrix. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-TIMP2 antibody [3A4] used in IHC (Paraffin sections) (IHC-P). GTX21828

GTX21828 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX21828 TIMP2 antibody [3A4].

Anti-TIMP2 antibody [3A4] used in Western Blot (WB). GTX21828

GTX21828 WB Image

Orthogonal

The data was published in the 2021 in Sci Rep. PMID: 33597628

REFERENCE

Application Reference

Wang J et al. Neoplasia 2019; 21 (9) : 908-920 p85α Inactivates MMP-2 and Suppresses Bladder Cancer Invasion by Inhibiting MMP-14 Transcription and TIMP-2 Degradation.
Application : WB
Reactivity : Human
Bu S et al. Arch Biochem Biophys 2014; Simulated physiological stretch-induced proliferation of human bladder smooth muscle cells is regulated by MMPs.
Application : WB
Reactivity : Human
Lin YC et al. PLoS One 2013; 8 (8) : e71903 Impact of flavonoids on matrix metalloproteinase secretion and invadopodia formation in highly invasive A431-III cancer cells.
Application : WB
Reactivity : Human
Corradi D et al. J Cardiovasc Electrophysiol 2012; 23 (3) : 271-9 Differential structural remodeling of the left-atrial posterior wall in patients affected by mitral regurgitation with or without persistent atrial fibrillation: a morphological and molecular study.
Application : IHC-P
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 319