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EPAS-1/HIF-2 alpha Antibody (190b): sc-13596
- EPAS-1/HIF-2 alpha Antibody (190b) is a mouse monoclonal IgG1 κ EPAS-1/HIF-2 alpha antibody, cited in 54 publications, provided at 200 µg/ml
- raised against recombinant EPAS-1 of human origin
- EPAS-1/HIF-2 alpha Antibody (190b) is recommended for detection of EPAS-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-EPAS-1/HIF-2 alpha Antibody (190b) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for Gel Supershift and ChIP applications (sc-13596 X, 200 µg/0.1 ml)
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of EPAS-1/HIF-2 alpha (190b): sc-13596.
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for EPAS-1/HIF-2 alpha Antibody (190b) for WB and IHC(P) applications. These reagents are now offered in bundles with EPAS-1/HIF-2 alpha Antibody (190b) (see ordering information below).
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EPAS-1 Antibody (190b) is an IgG1 κ mouse monoclonal EPAS-1 antibody (also designated EPAS1 antibody, HIF2A antibody, or HIF-2-alpha antibody) that detects the EPAS-1 protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. EPAS-1 Antibody (190b) is available as both the non-conjugated anti-EPAS-1 antibody form, as well as multiple conjugated forms of anti-EPAS-1 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Cell growth and viability is compromised by oxygen deprivation (hypoxia). Hypoxia-inducible factors, including HIF-1α, HIF-1β (also designated Arnt 1), EPAS-1 (also designated HIF-2α) and HIF-3α, induce glycolysis, erythropoiesis and angiogenesis in order to restore oxygen homeostasis. Hypoxia-inducible factors are members of the Per-Arnt-Sim (PAS) domain transcription factor family. In response to hypoxia, HIF-1α is upregulated and forms a heterodimer with Arnt 1 to form the HIF-1 complex. The HIF-1 complex recognizes and binds to the hypoxia responsive element (HRE) of hypoxia-inducible genes, thereby activating transcription. Hypoxia-inducible expression of some genes such as Glut-1, p53, p21 or Bcl-2, is HIF-1α dependent, whereas expression of others, such as p27, GADD 153 or HO-1, is HIF-1α independent. EPAS-1 and HIF-3α have also been shown to form heterodimeric complexes with Arnt 1 in response to hypoxia.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
References:
- Hypoxia-inducible factor 1 is a basic-helix-loop-helix-PAS heterodimer regulated by cellular O2 tension. | Wang, GL., et al. 1995. Proc Natl Acad Sci U S A. 92: 5510-4. PMID: 7539918
- Endothelial PAS domain protein 1 (EPAS1), a transcription factor selectively expressed in endothelial cells. | Tian, H., et al. 1997. Genes Dev. 11: 72-82. PMID: 9000051
- Molecular characterization of the murine Hif-1 alpha locus. | Luo, G., et al. 1997. Gene Expr. 6: 287-99. PMID: 9368100
- Selection and analysis of a mutant cell line defective in the hypoxia-inducible factor-1 alpha-subunit (HIF-1alpha). Characterization of hif-1alpha-dependent and -independent hypoxia-inducible gene expression. | Wood, SM., et al. 1998. J Biol Chem. 273: 8360-8. PMID: 9525945
- Role of HIF-1alpha in hypoxia-mediated apoptosis, cell proliferation and tumour angiogenesis. | Carmeliet, P., et al. 1998. Nature. 394: 485-90. PMID: 9697772
- Molecular characterization and chromosomal localization of a third alpha-class hypoxia inducible factor subunit, HIF3alpha. | Gu, YZ., et al. 1998. Gene Expr. 7: 205-13. PMID: 9840812