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CUL-1 Antibody (AS97): sc-12761
- CUL-1 Antibody (AS97) is a mouse monoclonal IgG2a κ, cited in 9 publications, provided at 200 µg/ml
- raised against recombinant Cul-1 of human origin
- Anti-CUL-1 Antibody (AS97) is recommended for detection of CUL-1 of mouse, rat and human origin by WB and IP
- Anti-CUL-1 Antibody (AS97) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CUL-1 (AS97): sc-12761.
- m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for CUL-1 Antibody (AS97) for WB applications. These reagents are now offered in bundles with CUL-1 Antibody (AS97) (see ordering information below).
CUL-1 Antibody (AS97) is an IgG2a κ mouse monoclonal CUL-1 antibody that detects CUL-1 of mouse, rat and human origin by WB and IP. CUL-1 Antibody (AS97) is available as the non-conjugated anti-CUL-1 antibody form, as well as multiple conjugated forms of anti-CUL-1 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Cullin proteins comprise a distinct family of mediators that participate in the selective targeting of proteins for ubiquitin (Ub)-mediated proteolysis. CUL-1, which is the mammalian homolog of yeast Cdc53, is an integral component of the E3 ubiquitin ligase complex designated SCF. The SCF (Skp1/CUL-1/F-box protein complex) consists of Skp1 associating with both CUL-1 and an F-box protein, such as Skp2, which determines the substrate specificity of the complex. CUL-1-mediated ubiquitination results in the degradation of cell cycle proteins cyclin D, p21 and cyclin E. Another cullin, CUL-3, facilitates the degradation of cyclin E independent of SCF activity, while CUL-2 associates with the tumor suppressing protein VHL and elongin B to form VBC complexes, which structurally resemble the SCF ligase. Proteolysis also occurs by way of CUL-4 associating with Nedd-8, a ubiquitin-like protein, where it too functions as an active component of a multifunctional E3 complex. CUL-5, also designated vasopressin-activated, calcium-mobilizing protein (VACM-1), is also included in the cullin family as it shares substantial sequence homology with CUL-1.
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