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p-Pol II Antibody (8A7) - Western Blotting - Image 1259

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p-Pol II Antibody (8A7): sc-13583. Western blot analysis of Pol II phosphorylation in untreated (A), heat shocked (C) and calyculin-treated (E) HeLa cultures. Total Pol II levels were determined in the same respective extracts by using Pol II (A-10): sc-17798 (B,D,F).

p-Pol II Antibody (8A7): sc-13583

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Datasheets
  • p-Pol II Antibody (8A7) is a mouse monoclonal IgM κ, cited in 26 publications, provided at 200 µg/ml
  • raised against a synthetic peptide containing phosphorylated Ser 1801 of p-Pol II of human origin
  • recommended for detection of Ser 1801 phosphorylated RNA polymerase II of human origin and correspondingly phosphorylated RNA polymerase II of mouse, rat and S. cerevisiae origin by WB, IP, IF and IHC(P)
  • TransCruz reagent for Gel Supershift and ChIP applications (sc-13583 X, 200 µg/0.1 ml)
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for p-Pol II Antibody (8A7). This work is in progress.

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RNA polymerase II (Pol II) is a multi-subunit enzyme responsible for the transcription of protein-coding genes. Transcription initiation requires recruitment of the complete transcription machinery to a promoter via solicitation by activators and chromatin remodeling factors. Pol II can coordinate 10 to 14 subunits. This complex interacts with the promoter regions of genes and a variety of elements and transcription factors. The DNA binding domain of the polymerase is a groove where TFIIB orients the DNA for unwinding and transcription.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

References:

  1. Structural basis of transcription: an RNA polymerase II-TFIIB cocrystal at 4.5 Angstroms.  |  Bushnell, DA., et al. 2004. Science. 303: 983-8. PMID: 14963322
  2. Structure and function of RNA polymerase II.  |  Cramer, P. 2004. Adv Protein Chem. 67: 1-42. PMID: 14969722
  3. Mechanism of RNA polymerase I transcription.  |  Comai, L. 2004. Adv Protein Chem. 67: 123-55. PMID: 14969726
  4. The RNA polymerase II transcription cycle: cycling through chromatin.  |  Svejstrup, JQ. 2004. Biochim Biophys Acta. 1677: 64-73. PMID: 15020047
  5. RNA polymerase III transcription and cancer.  |  White, RJ. 2004. Oncogene. 23: 3208-16. PMID: 15094770
  6. BRCA1 cooperates with NUFIP and P-TEFb to activate transcription by RNA polymerase II.  |  Cabart, P., et al. 2004. Oncogene. 23: 5316-29. PMID: 15107825
  7. Epidermal growth factor enhances cellular TATA binding protein levels and induces RNA polymerase I- and III-dependent gene activity.  |  Zhong, S., et al. 2004. Mol Cell Biol. 24: 5119-29. PMID: 15169879
  8. Distinct mechanisms for repression of RNA polymerase III transcription by the retinoblastoma tumor suppressor protein.  |  Hirsch, HA., et al. 2004. Mol Cell Biol. 24: 5989-99. PMID: 15199152
  9. Downstream DNA selectively affects a paused conformation of human RNA polymerase II.  |  Palangat, M., et al. 2004. J Mol Biol. 341: 429-42. PMID: 15276834

When should the TransCruz reagent sc-13583 X be used instead of the standard form sc-13583?

Asked by: Trav11
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by Todd from normal use During normal use, the traits in WB are good. The mutation state in the reference object is not obvious
Date published: 2017-01-18
Rated 4 out of 5 by Gina from Pretty good antibody Pretty good antibody. We have used a different lot in the past and it worked really well. No big complaints.
Date published: 2015-06-19
Rated 5 out of 5 by fm from Use it routinely Use it routinely, works well in WB, no complaints
Date published: 2015-04-21
Rated 5 out of 5 by NZ from Strong nuclear localization in IF in methanol Strong nuclear localization in IF in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-04-08
Rated 5 out of 5 by JT from Worked well in IF in monkey CV Worked well in IF in monkey CV-1 cells. -SCBT Publication Review
Date published: 2015-03-05
Rated 5 out of 5 by SM from Good antibody for ChIP in human HEK293 Good antibody for ChIP in human HEK293, MCF-7 cells. -SCBT Publication Review
Date published: 2015-01-14
Rated 5 out of 5 by MC from Strong band in WB in HeLa whole cell lysates Strong band in WB in HeLa whole cell lysates. -SCBT QC
Date published: 2013-08-20
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p-Pol II Antibody (8A7) is rated 4.9 out of 5 by 7.
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