Cdc27 Antibody (AF3.1): sc-9972

Cdc27 Antibody (AF3.1) is an IgG_2b κ mouse monoclonal Cdc27 antibody (also designated H-NUC antibody, Anaphase-promoting complex subunit 3 antibody or ANAPC3 antibody) that detects the Cdc27 protein of mouse, rat, human and Xenopus laevis origin by WB, IP, IF and IHC(P). Cdc27 Antibody (AF3.1) is available as both the non-conjugated anti-Cdc27 antibody form, as well as multiple conjugated forms of anti-Cdc27 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor^® conjugates. Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by the proteolysis of cyclins. The cell division cycle (Cdc) genes are required at various points in the cell cycle. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. Cdc6 is the human homolog of Saccharomyces cerevisiae Cdc6, which is involved in the initiation of DNA replication. Cdc37 appears to facilitate Cdk4/cyclin D1 complex formation and has been shown to form a stable complex with Hsp90. Cdc34, Cdc27 and Cdc16 function as ubiquitin-conjugating enzymes. Cdc34 is thought to be the structural and functional homolog of Saccharomyces cerevisiae Cdc34, which is essential for the G1 to S phase transition. Cdc16 and Cdc27 are components of the APC (anaphase-promoting complex) which ubiquitinates cyclin B, resulting in cyclin B/Cdk complex degradation.

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LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

References:

1. Depletion of anaphase-promoting complex or cyclosome (APC/C) subunit homolog APC1 or CDC27 of Trypanosoma brucei arrests the procyclic form in metaphase but the bloodstream form in anaphase.  |  Kumar, P. and Wang, CC. 2005. J Biol Chem. 280: 31783-91. PMID: 15994309
2. RNA interference knockdown of hU2AF35 impairs cell cycle progression and modulates alternative splicing of Cdc25 transcripts.  |  Pacheco, TR., et al. 2006. Mol Biol Cell. 17: 4187-99. PMID: 16855028
3. cdc25 is a specific tyrosine phosphatase that directly activates p34cdc2.  |  Gautier, J., et al. 1991. Cell. 67: 197-211. PMID: 1913817
4. Mitotic transmission of artificial chromosomes in cdc mutants of the yeast, Saccharomyces cerevisiae.  |  Palmer, RE., et al. 1990. Genetics. 125: 763-74. PMID: 2204582
5. A new twist to the cell cycle.  |  Barinaga, M. 1995. Science. 269: 631-2. PMID: 7624789
6. A 20S complex containing CDC27 and CDC16 catalyzes the mitosis-specific conjugation of ubiquitin to cyclin B.  |  King, RW., et al. 1995. Cell. 81: 279-88. PMID: 7736580
7. Cloning of the human homolog of the CDC34 cell cycle gene by complementation in yeast.  |  Plon, SE., et al. 1993. Proc Natl Acad Sci U S A. 90: 10484-8. PMID: 8248134
8. Mammalian p50Cdc37 is a protein kinase-targeting subunit of Hsp90 that binds and stabilizes Cdk4.  |  Stepanova, L., et al. 1996. Genes Dev. 10: 1491-502. PMID: 8666233
9. A human protein related to yeast Cdc6p.  |  Williams, RS., et al. 1997. Proc Natl Acad Sci U S A. 94: 142-7. PMID: 8990175

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Cdc27 Antibody (AF3.1) has 71 citations in a variety of scientific publications.

Primary antibodies like Anti-Cdc27 Antibody (AF3.1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Cdc27 Antibody (AF3.1) Specifications

Alternate Product Name

Cdc27

Product Clone Name

AF3.1

Classification

Primary Antibody

Clonality

Monoclonal Antibody

Antibody Isotype

IgG_2b kappa light chain

Amino Acids

814-823

Epitope Target

Cdc27

Epitope Species

human

Product Reactivity/Detection

Cdc27

Species Reactivity/Detection

mouse, rat, human

Additional Mammalian Reactivity

equine and canine

Additional Non-Mammalian Reactivity

Xenopus

Applications

WB, IP, IF, IHC(P)

Vialing Information

Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations

71 citations in various scientific publications

Recommended Secondary Detection Reagents


Mouse IgG_2b Binding Proteins

• m-IgG_2b BP-HRP: sc-542741 (Mouse IgG_2b binding protein conjugated to horseradish peroxidase)
• m-IgG_2b BP-B: sc-542744 (Mouse IgG_2b binding protein conjugated to biotin)
• m-IgG_2b BP-FITC: sc-542743 (Mouse IgG_2b binding protein conjugated to fluorescein isothiocyanate)
• m-IgG_2b BP-CFL 488: sc-542745 (Mouse IgG_2b binding protein conjugated to CruzFluor™ 488)
• m-IgG_2b BP-CFL 555: sc-542746 (Mouse IgG_2b binding protein conjugated to CruzFluor™ 555)
• m-IgG_2b BP-CFL 594: sc-542747 (Mouse IgG_2b binding protein conjugated to CruzFluor™ 594)
• m-IgG_2b BP-CFL 647: sc-542748 (Mouse IgG_2b binding protein conjugated to CruzFluor™ 647)
• m-IgG_2b BP-CFL 680: sc-542749 (Mouse IgG_2b binding protein conjugated to CruzFluor™ 680)
• m-IgG_2b BP-CFL 790: sc-542750 (Mouse IgG_2b binding protein conjugated to CruzFluor™ 790)
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Mouse IgG Kappa Light Chain Binding Proteins
• m-IgGκ BP-HRP: sc-516102 (Mouse IgGκ light chain binding protein conjugated to horseradish peroxidase)
• m-IgGκ BP-B: sc-516142 (Mouse IgGκ light chain binding protein conjugated to biotin)
• m-IgGκ BP-FITC: sc-516140 (Mouse IgGκ light chain binding protein conjugated to fluorescein isothiocyanate)
• m-IgGκ BP-PE: sc-516141 (Mouse IgGκ light chain binding protein conjugated to phycoerythrin)
• m-IgGκ BP-CFL 488: sc-516176 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 488)
• m-IgGκ BP-CFL 555: sc-516177 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 555)
• m-IgGκ BP-CFL 594: sc-516178 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 594)
• m-IgGκ BP-CFL 647: sc-516179 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 647)
• m-IgGκ BP-CFL 680: sc-516180 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 680)
• m-IgGκ BP-CFL 790: sc-516181 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 790)



Available Conjugates/Forms




AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin




Primary antibodies for non-mammalian targets are useful in detecting proteins of plant, viral, bacterial, avian, fish, yeast and invertebrates. Some examples include Drosophila, zebrafish, Xenopus, Arabidopsis, C. elegans, Clostridium, Campylobacter, Heliobacter pylori, Hepatitis, HIV, HPV, HSV, Influenza, Klebsiella, M. tuberculosis, Plasmodium, Pseudomonas, RSV, Saccharomyces cerevisiae, Salmonella, Schistosoma, Shigella and Staphylococcus.

Santa Cruz Biotechnology monoclonal antibodies can be used in assays including flow cytometry, immunoprecipitation, Western blot analysis, and immunostaining. Anti-Cdc27 Antibody (AF3.1) is recommended for use in the following applications: WB (Western blotting), IP (Immunoprecipitation), IF (Immunofluorescence), IHC(P) (Immunohistochemistry - Paraffin Embedded).

Mouse secondary antibodies, control sera and control immunoglobulins are also offered to be used in combination with our primary monoclonal antibodies.

Tissue extracts, nuclear extracts and whole cell lysates are used with Santa Cruz Biotechnology monoclonal antibodies for western blotting or immunoprecipitation positive controls. Nuclear extracts are ideal for western blot analysis or gel shift assays positive controls. Phospho-enriched whole cell lysates and tissue extracts are available for phospho-specific antibody positive controls.

Monoclonal secondary antibodies are offered in various conjugated forms, including: Biotin (B), CruzFluor™ 790, 680, 647, 594, 555, and 488, FITC, HRP, PerCP, PerCP-Cy5.5, Texas Red (TR), and TRITC (tetramethyl rhodamine isothiocyanate) (R), for research applications like Flow Cytometry (FCM), Immunofluorescence (IF), Immunohistochemistry (IHC) staining and Western Blotting (WB). Not intended for diagnostic or therapeutic use.

Affinity purified immunoglobulins (IgGs)—conjugated and unconjugated—are available as negative controls for flow cytometry (FCM), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP) and Western Blotting (WB) applications.

Normal sera for immunohistochemistry (IHC) applications are available as neat serum for blocking reagents. The serum which is used should match the species of the secondary antibody.