CD8 Antibody (UCH-T4): sc-1181

CD8 Antibody (UCH-T4) is an IgG2a κ mouse monoclonal CD8 antibody that detects CD8 of human origin by IP, IF, IHC(P) and FCM. CD8 Antibody (UCH-T4) is available as the non-conjugated anti-CD8 antibody form, as well as multiple conjugated forms of anti-CD8 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor^® conjugates. The T cell receptor (TCR) is a heterodimer composed of either α and β or γ and δ chains. CD3 chains and the CD4 or CD8 co-receptors are also required for efficient signal transduction through the TCR. The TCR is expressed on T helper and T cytotoxic cells that can be distinguished by their expression of CD4 and CD8; T helper cells express CD4 proteins and T cytotoxic cells display CD8. CD8 (also designated Leu 2 or T8), a cell surface glycoprotein, is a two chain complex (αα or αβ) receptor that binds class I MHC molecules presented by the antigen-presenting cell (APC). A primary function of CD8 is to facilitate antigen recognition by the TCR and to strengthen the avidity of the TCR-antigen interactions. An additional role for CD8-expressing T cells may be to maintain low levels of HIV expression.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

References:

1. The T cell receptor as a multicomponent signalling machine: CD4/CD8 coreceptors and CD45 in T cell activation.  |  Janeway, CA. 1992. Annu Rev Immunol. 10: 645-74. PMID: 1534242
2. The immunobiology of T cells with invariant gamma delta antigen receptors.  |  Allison, JP. and Havran, WL. 1991. Annu Rev Immunol. 9: 679-705. PMID: 1832874
3. CD4 and CD8 act as co-receptors during thymic selection of the T cell repertoire.  |  Zúηiga-Pflυcker, JC., et al. 1991. Semin Immunol. 3: 167-75. PMID: 1909594
4. CD4 and CD8 recognition of class II and class I molecules of the major histocompatibility complex.  |  Fleury, SG., et al. 1991. Semin Immunol. 3: 177-85. PMID: 1909595
5. Structure and expression of the gene encoding CD8 alpha chain (Leu-2/T8).  |  Nakayama, K., et al. 1989. Immunogenetics. 30: 393-7. PMID: 2509342
6. HIV-specific CD8+ T-cell immune responses and viral replication.  |  Buseyne, F. and Rivière, Y. 1993. AIDS. 7 Suppl 2: S81-5. PMID: 7909230
7. T cell receptor interaction with peptide/major histocompatibility complex (MHC) and superantigen/MHC ligands is dominated by antigen.  |  Ehrich, EW., et al. 1993. J Exp Med. 178: 713-22. PMID: 8393480
8. Distinct roles for CD4 and CD8 as co-receptors in antigen receptor signalling.  |  Julius, M., et al. 1993. Immunol Today. 14: 177-83. PMID: 8499078
9. A novel leukocyte adhesion deficiency caused by expressed but nonfunctional beta2 integrins Mac-1 and LFA-1.  |  Hogg, N., et al. 1999. J Clin Invest. 103: 97-106. PMID: 9884339

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CD8 Antibody (UCH-T4) has 15 citations in a variety of scientific publications.

Primary antibodies like Anti-CD8 Antibody (UCH-T4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CD8 Antibody (UCH-T4) Specifications

Alternate Product Name

CD8

Product Clone Name

UCH-T4

Classification

Primary Antibody

Clonality

Monoclonal Antibody

Antibody Isotype

IgG_2a kappa light chain

Amino Acids

Epitope Target

CD8

Epitope Species

human

Product Reactivity/Detection

CD8

Species Reactivity/Detection

human

Additional Mammalian Reactivity

N/A

Additional Non-Mammalian Reactivity

N/A

Applications

IP, IF, IHC(P), FCM

Vialing Information

Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations

15 citations in various scientific publications

Recommended Secondary Detection Reagents

Mouse IgG Fc Binding Proteins

• m-IgG Fc BP-HRP: sc-525409 (Mouse IgG Fc binding protein conjugated to horseradish peroxidase)
• m-IgG Fc BP-B: sc-533652 (Mouse IgG Fc binding protein conjugated to biotin)
• m-IgG Fc BP-FITC: sc-533651 (Mouse IgG Fc binding protein conjugated to fluorescein isothiocyanate)
• m-IgG Fc BP-CFL 488: sc-533653 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 488)
• m-IgG Fc BP-CFL 555: sc-533654 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 555)
• m-IgG Fc BP-CFL 594: sc-533655 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 594)
• m-IgG Fc BP-CFL 647: sc-533656 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 647)
• m-IgG Fc BP-CFL 680: sc-533657 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 680)
• m-IgG Fc BP-CFL 790: sc-533658 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 790)

• m-IgG_2a BP-HRP: sc-542731 (Mouse IgG_2a binding protein conjugated to horseradish peroxidase)
• m-IgG_2a BP-B: sc-542734 (Mouse IgG_2a binding protein conjugated to biotin)
• m-IgG_2a BP-FITC: sc-542733 (Mouse IgG_2a binding protein conjugated to fluorescein isothiocyanate)
• m-IgG_2a BP-CFL 488: sc-542735 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 488)
• m-IgG_2a BP-CFL 555: sc-542736 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 555)
• m-IgG_2a BP-CFL 594: sc-542737 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 594)
• m-IgG_2a BP-CFL 647: sc-542738 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 647)
• m-IgG_2a BP-CFL 680: sc-542739 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 680)
• m-IgG_2a BP-CFL 790: sc-542740 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 790)

Available Conjugates/Forms

AC: Agarose, AF405: Alexa Fluor^® 405, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin