CD5 Antibody (UCH-T2): sc-1180

CD5 Antibody (UCH-T2) is an IgG₁ κ mouse monoclonal CD5 antibody (also designated CD5 antibody) that detects the CD5 protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM. CD5 Antibody (UCH-T2) is available as both the non-conjugated anti-CD5 antibody form, as well as multiple conjugated forms of anti-CD5 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor^® conjugates. CD5 (also designated Lyt-1) has been identified as a transmembrane glycoprotein that is expressed on 70% of normal peripheral blood lymphocytes and on virtually all T lymphocytes in thymus and peripheral blood. Activation of T cells through the T cell receptor (TCR) results in tyrosine phosphorylation of CD5, and the absence of CD5 renders T cells hyper-responsive to TCR-mediated activation. CD5 associates with the TCR/ CD3-z chain and with the Src family kinase Lck p56. In vitro studies have shown a 10- to 15-fold increase in the kinase activity of Lck bound to CD5. The B cell antigen, CD72, serves as a receptor for CD5. The consequence of CD5 binding to its cognate receptor is still in question and likely plays a role in thymic selection.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

References:

1. CD5 is phosphorylated on tyrosine after stimulation of the T-cell antigen receptor complex.  |  Davies, AA., et al. 1992. Proc Natl Acad Sci U S A. 89: 6368-72. PMID: 1378627
2. CD5+ B cells and the immune system.  |  Lydyard, PM., et al. 1993. Immunol Lett. 38: 159-66. PMID: 7507466
3. The T-cell antigen CD5 acts as a receptor and substrate for the protein-tyrosine kinase p56lck.  |  Raab, M., et al. 1994. Mol Cell Biol. 14: 2862-70. PMID: 7513045
4. Anti-CD5 therapy decreases severity of established disease in collagen type II-induced arthritis in DBA/1 mice.  |  Plater-Zyberk, C., et al. 1994. Clin Exp Immunol. 98: 442-7. PMID: 7527741
5. A role for CD5 in TCR-mediated signal transduction and thymocyte selection.  |  Tarakhovsky, A., et al. 1995. Science. 269: 535-7. PMID: 7542801
6. CD5 expression on B cells may be an activation marker for secretion of anti-myelin antibodies in patients with polyneuropathy associated with monoclonal gammopathy.  |  Ekerfelt, C., et al. 1995. Clin Exp Immunol. 101: 346-50. PMID: 7544252
7. Expression of CD5 and CD72 on T and B cell subsets in rheumatoid arthritis and Sjögren′s syndrome.  |  Jamin, C., et al. 1993. Clin Exp Immunol. 92: 245-50. PMID: 7683586
8. Detection of T and B cells in many animal species using cross-reactive anti-peptide antibodies.  |  Jones, M., et al. 1993. J Immunol. 150: 5429-35. PMID: 8515069
9. The structure of avian CD5 implies a conserved function.  |  Koskinen, R., et al. 1998. J Immunol. 160: 4943-50. PMID: 9590242

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CD5 Antibody (UCH-T2) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-CD5 Antibody (UCH-T2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CD5 Antibody (UCH-T2) Specifications

Alternate Product Name

CD5

Product Clone Name

UCH-T2

Classification

Primary Antibody

Clonality

Monoclonal Antibody

Antibody Isotype

IgG₁ kappa light chain

Amino Acids

Epitope Target

CD5

Epitope Species

human

Product Reactivity/Detection

CD5

Species Reactivity/Detection

mouse, rat, human

Additional Mammalian Reactivity

N/A

Additional Non-Mammalian Reactivity

N/A

Applications

WB, IP, IF, IHC(P), FCM

Vialing Information

Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations

4 citations in various scientific publications

Recommended Secondary Detection Reagents

Mouse IgG Fc Binding Proteins

• m-IgG Fc BP-HRP: sc-525409 (Mouse IgG Fc binding protein conjugated to horseradish peroxidase)
• m-IgG Fc BP-B: sc-533652 (Mouse IgG Fc binding protein conjugated to biotin)
• m-IgG Fc BP-FITC: sc-533651 (Mouse IgG Fc binding protein conjugated to fluorescein isothiocyanate)
• m-IgG Fc BP-CFL 488: sc-533653 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 488)
• m-IgG Fc BP-CFL 555: sc-533654 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 555)
• m-IgG Fc BP-CFL 594: sc-533655 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 594)
• m-IgG Fc BP-CFL 647: sc-533656 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 647)
• m-IgG Fc BP-CFL 680: sc-533657 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 680)
• m-IgG Fc BP-CFL 790: sc-533658 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 790)

• m-IgG₁ BP-HRP: sc-525408 (Mouse IgG₁ binding protein conjugated to horseradish peroxidase)
• m-IgG₁ BP-B: sc-533660 (Mouse IgG₁ binding protein conjugated to biotin)
• m-IgG₁ BP-FITC: sc-533659 (Mouse IgG₁ binding protein conjugated to fluorescein isothiocyanate)
• m-IgG₁ BP-CFL 488: sc-533661 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 488)
• m-IgG₁ BP-CFL 555: sc-533662 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 555)
• m-IgG₁ BP-CFL 594: sc-533663 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 594)
• m-IgG₁ BP-CFL 647: sc-533664 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 647)
• m-IgG₁ BP-CFL 680: sc-533665 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 680)
• m-IgG₁ BP-CFL 790: sc-533666 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 790)

• m-IgGκ BP-HRP: sc-516102 (Mouse IgGκ light chain binding protein conjugated to horseradish peroxidase)
• m-IgGκ BP-B: sc-516142 (Mouse IgGκ light chain binding protein conjugated to biotin)
• m-IgGκ BP-FITC: sc-516140 (Mouse IgGκ light chain binding protein conjugated to fluorescein isothiocyanate)
• m-IgGκ BP-PE: sc-516141 (Mouse IgGκ light chain binding protein conjugated to phycoerythrin)
• m-IgGκ BP-CFL 488: sc-516176 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 488)
• m-IgGκ BP-CFL 555: sc-516177 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 555)
• m-IgGκ BP-CFL 594: sc-516178 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 594)
• m-IgGκ BP-CFL 647: sc-516179 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 647)
• m-IgGκ BP-CFL 680: sc-516180 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 680)
• m-IgGκ BP-CFL 790: sc-516181 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 790)

Available Conjugates/Forms

AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin