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CD14 Antibody (UCH-M1) - Western Blotting - Image 4742

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CD14 Antibody (UCH-M1): sc-1182. Western blot analysis of CD14 expression in BJAB whole cell lysate.

CD14 Antibody (UCH-M1): sc-1182

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Datasheets
  • CD14 Antibody (UCH-M1) is a mouse monoclonal IgG2a κ, cited in 46 publications, provided at 200 µg/ml
  • raised against human thymocytes/Sezary T cells
  • CD14 Antibody (UCH-M1) is recommended for detection of CD14 monocyte specific cell surface protein of human origin by WB, IP, IF, IHC(P) and FCM
  • Anti-CD14 Antibody (UCH-M1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CD14 (UCH-M1): sc-1182.
  • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for CD14 Antibody (UCH-M1) for WB and IHC(P) applications. These reagents are now offered in bundles with CD14 Antibody (UCH-M1) (see ordering information below).

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CD14 Antibody (UCH-M1) is an IgG2a κ mouse monoclonal CD14 antibody that detects CD14 monocyte specific cell surface protein of human origin by WB, IP, IF, IHC(P) and FCM. CD14 Antibody (UCH-M1) is available as the non-conjugated anti-CD14 antibody form, as well as multiple conjugated forms of anti-CD14 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Lipopolysaccharide (LPS) elicits the secretion of mediators and cytokines produced by activated macrophages and monocytes. CD14 is a glycosylphosphatidylinositol (GPI)-anchored protein found on the surfaces of monocytes and polymorphonuclear leukocytes. CD14 functions as a receptor for LPS, resulting in the secretion of various proteins. An important component in the LPS activation of monocytes through the CD14 receptor is the "adapter molecule," lipopolysaccharide binding protein (LBP). There are two forms of CD14, a membrane-associated form (mCD14), and a soluble form (sCD14). mCD14 responds to LPS alone and facilitates the secretion of proteins, while cells not expressing mCD14 fail to respond to LPS. The cells that lack mCD14 respond to LPS/LBP in the presence of sCD14.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

References:

  1. Function of lipopolysaccharide (LPS)-binding protein (LBP) and CD14, the receptor for LPS/LBP complexes: a short review.  |  Schumann, RR. 1992. Res Immunol. 143: 11-5. PMID: 1373512
  2. Monocyte antigen CD14 is a phospholipid anchored membrane protein.  |  Simmons, DL., et al. 1989. Blood. 73: 284-9. PMID: 2462937
  3. Soluble lipopolysaccharide receptor (CD14) is released via two different mechanisms from human monocytes and CD14 transfectants.  |  Bufler, P., et al. 1995. Eur J Immunol. 25: 604-10. PMID: 7533093
  4. Lipopolysaccharide binding protein and CD14 modulate the synthesis of platelet-activating factor by human monocytes and mesangial and endothelial cells stimulated with lipopolysaccharide.  |  Camussi, G., et al. 1995. J Immunol. 155: 316-24. PMID: 7541418
  5. CD14 and other recognition molecules for lipopolysaccharide: a review.  |  Kielian, TL. and Blecha, F. 1995. Immunopharmacology. 29: 187-205. PMID: 7542643
  6. Neutrophil response to endotoxin in the adult respiratory distress syndrome: role of CD14.  |  Parsons, PE., et al. 1995. Am J Respir Cell Mol Biol. 13: 152-60. PMID: 7542895
  7. Endotoxin receptors (CD14) are found with CD16 (Fc gamma RIII) in an intracellular compartment of neutrophils that contains alkaline phosphatase.  |  Detmers, PA., et al. 1995. J Immunol. 155: 2085-95. PMID: 7543538

What is the CD14 concentration used for flow cytometry?

Asked by: Bravo_biologist
Thank you for your question. You can find our recommended protocol for flow cytometry under this link: https://www.scbt.com/resources/protocols/flow-cytometry. We recommend using 1 µg of fluorochrome-conjugated antibodies per 1 million cells in 100 µl buffer per tube.
Answered by: Tech Support Europe
Date published: 2023-03-21

What negative control should be used when using the conjugated version of CD14 (UCH-M1): sc-1182 monoclonal antibody in flow cytometry?

Asked by: jenniferc
Thank you for your inquiry. We recommend using the isotype specific normal mouse IgG conjugated to the same fluorophore. For example, if you are using sc-1182 PE, then you would want to use normal mouse IgG2a PE, sc-2867.
Answered by: Technical Support 9
Date published: 2017-01-11

What secondary is best to use with CD14 (UCH-M1): sc-1182 monoclonal antibody?

Asked by: jenniferc
Thank you for your question. While most anti-mouse IgG secondary antibodies are appropriate for use with CD14 (UCH-M1): sc-1182, we recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 9
Date published: 2017-01-11
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Rated 4 out of 5 by Valeria92 from good antibody for IF I used this antibody in IF in frozen human colon tissue sections and it works very well.
Date published: 2018-06-20
Rated 5 out of 5 by Bill28 from Outstanding Great western blot data; strong signal, lowbackground; also superb FCM and IHC results
Date published: 2017-01-28
Rated 5 out of 5 by Trav11 from Great, versatile antibody The CD14 Antibody (UCH-M1): sc-1182 works well for a variety of applications including WB, IF, IHC(P) and FCM. This antibody has also been cited over 30 times for further verification.
Date published: 2017-01-12
Rated 5 out of 5 by LeeK from works great for IF Used it at 1:50-1:100 dilution on PBMCs for 1hr at RT. Worked great, positive cells very bright with no non-specific binding.
Date published: 2016-11-07
Rated 1 out of 5 by Brian from tested in ASTMA tested in ASTMA-23, and Lymph TMA tissue for IHC. Negative @1:25; Not a Good Antibody for IHC
Date published: 2016-06-13
Rated 4 out of 5 by MS from Various publishable data in FCM using human Various publishable data in FCM using human peripheal blood, THP-1 and PBMC cell lines. -SCBT Publication Review
Date published: 2015-05-19
Rated 4 out of 5 by ET from Various publishable data in WB using human Various publishable data in WB using human LPS and PGN stimulated uroepithelial cell lines T24, 5637, UM-UC-3, and HT1197, transf. K562 and Human oviduct cell lines. -SCBT Publication Review
Date published: 2015-03-01
Rated 4 out of 5 by SS from Great immunoperoxidase membrane staining Great immunoperoxidase membrane staining in formalin-fixed, paraffin-embedded normal human lymph node. -SCBT QC
Date published: 2015-02-24
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CD14 Antibody (UCH-M1) is rated 4.0 out of 5 by 12.
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