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involucrin Antibody (SY5): sc-21748
- involucrin Antibody (SY5) is a mouse monoclonal IgG1 involucrin antibody, cited in 59 publications, provided at 200 µg/ml
- raised against pure involucrin from human keratinocytes
- involucrin Antibody (SY5) is recommended for detection of involucrin of mouse, rat and human origin by WB, IP, IF and IHC(P)
- Anti-involucrin Antibody (SY5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of involucrin (SY5): sc-21748.
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for involucrin Antibody (SY5). This work is in progress.
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involucrin Antibody (SY5) is an IgG1 mouse monoclonal involucrin antibody (also designated IVL antibody) that detects the involucrin protein of mouse, rat and human origin by WB, IP, IF and IHC(P). involucrin Antibody (SY5) is available as both the non-conjugated anti-involucrin antibody form, as well as multiple conjugated forms of anti-involucrin antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Involucrin is a precursor protein of the keratinocyte cornified envelope, which is formed beneath the inner surface of the cell membrane during terminal differentiation. Involucrin first appears in the cell cytosol but ultimately becomes cross-linked to membrane proteins by transglutaminase. During keratinocyte terminal differentiation, glutamine residues of involucrin become covalently cross-linked to other envelope precursors via covalent ε-(γ-glutamyl) lysine bonds. Moreover, its large size allows involucrin to cross-link molecules that are separated by substantial distances in the cornified envelope. These properties allow a single involucrin molecule to form multiple cross-links, in multiple spatial planes, with other envelope precursors. Involucrin is specifically expressed in Chinese hamster ovarian cells (fibroblasts), PtK2 rat kangaroo kidney cells (simple epithelial), and rat epidermal keratinocytes (stratifying squamous epithelial).
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
References:
- Biophysical characterization of involucrin reveals a molecule ideally suited to function as an intermolecular cross-bridge of the keratinocyte cornified envelope. | Yaffe, MB., et al. 1992. J Biol Chem. 267: 12233-8. PMID: 1601889
- Stable expression of transfected human involucrin gene in various cell types: evidence for in situ cross-linking by type I and type II transglutaminase. | Rorke, EA. and Eckert, RL. 1991. J Invest Dermatol. 97: 543-8. PMID: 1678767
- Structure and evolution of the human involucrin gene. | Eckert, RL. and Green, H. 1986. Cell. 46: 583-9. PMID: 2873896
- Tissue-specific and differentiation-appropriate expression of the human involucrin gene in transgenic mice: an abnormal epidermal phenotype. | Crish, JF., et al. 1993. Differentiation. 53: 191-200. PMID: 8405770
- Repression of involucrin gene expression by transcriptional enhancer factor 1 (TEF-1). | Takahashi, H., et al. 1995. Arch Dermatol Res. 287: 740-6. PMID: 8554386