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ErbB3/HER3 Antibody (RTJ.2): sc-415

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Datasheets
  • ErbB3/HER3 Antibody (RTJ.2) is a mouse monoclonal IgG1 κ, cited in 59 publications, provided at 200 µg/ml
  • epitope mapping within the cytoplasmic domain of ErbB-3 of human origin
  • Anti-ErbB3/HER3 Antibody (RTJ.2) is recommended for detection of ErbB-3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM
  • Anti-ErbB3/HER3 Antibody (RTJ.2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ErbB3/HER3 (RTJ.2): sc-415.
  • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for ErbB3/HER3 Antibody (RTJ.2) for WB and IHC(P) applications. These reagents are now offered in bundles with ErbB3/HER3 Antibody (RTJ.2) (see ordering information below).

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ErbB3/HER3 Antibody (RTJ.2) is an IgG1 κ mouse monoclonal ErbB-3 antibody (also designated ErbB-3 antibody) that detects ErbB-3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM. ErbB3/HER3 Antibody (RTJ.2) is available as the non-conjugated anti-ErbB-3 antibody form, as well as multiple conjugated forms of anti-ErbB-3 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. The EGF receptor family comprises several related receptor tyrosine kinases that are frequently overexpressed in a variety of carcinomas. Members of this receptor family include EGFR (HER1), Neu (ErbB-2, HER2), ErbB-3 (HER3) and ErbB-4 (HER4), which form either homodimers or heterodimers upon ligand binding. Full length ErbB-3 is overexpressed in human mammary tumors. The ErbB-3 gene also produces several alternative variants, including a secreted form of which negatively regulates heregulin stimulated ErbB activation. ErbB-3 heterodimerizes with Neu and binds heregulin in order to activate phosphoinositide (PI) 3-kinase. The recruitment and activation of PI 3-kinase occurs via its interaction with phosphorylated YXXM motifs in the carboxy terminus of ErbB-3.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

Can ErbB-3 (RTJ.2): sc-415 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: cjMara
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, ErbB-3 (RTJ.2): sc-415 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by JM from Published results of immunoprecipitation Published results of immunoprecipitation using human lung cancer cell lines. -SCBT Publication Review
Date published: 2015-05-18
Rated 5 out of 5 by MC from Worked well for IHC pn CJ7 embryonic stem cells Worked well for IHC pn CJ7 embryonic stem cells. -SCBT Publication Review
Date published: 2015-04-21
Rated 5 out of 5 by SS from Exceptional immunofluorescence membrane staining Exceptional immunofluorescence membrane staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-03-15
Rated 5 out of 5 by CJ from Published immunohistochemical analysis of Published immunohistochemical analysis of paraffin embedded non-neoplastic urothelium and chronic pyelonephritis chronic ureteritis slices, kidney laceration slices and uretero-pelvic junction stenosis sections. -SCBT Publication Review
Date published: 2015-02-05
Rated 5 out of 5 by FL from Robust signal in Western blot analysis of ErbB Robust signal in Western blot analysis of ErbB-3 expression in SK-BR-3 whole cell lysate. -SCBT QC
Date published: 2015-01-03
Rated 5 out of 5 by ET from Exceptional intracellular FCM data of fixed Exceptional intracellular FCM data of fixed and permeabilized HEL 92.1.7 cells. -SCBT QC
Date published: 2013-12-12
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ErbB3/HER3 Antibody (RTJ.2) is rated 5.0 out of 5 by 6.
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