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p53 Antibody (DO-1) - Western Blotting - Image 385951

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Simultaneous direct near-infrared western blot analysis of p53 expression, detected with p53 Antibody (DO-1) AF680: sc-126 AF680 and GAPDH expression, detected with GAPDH (0411) Alexa Fluor® 790: sc-47724 AF790 in A-431 (A) and SW480 (B) whole cell lysates. Blocked with UltraCruz® Blocking Reagent: sc-516214.

p53 Antibody (DO-1): sc-126

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Datasheets
  • p53 Antibody (DO-1) is a mouse monoclonal IgG2a κ p53 antibody, cited in 6,419 publications, provided at 200 µg/ml
  • N-terminal epitope mapping between amino acid residues 11-25 of p53 of human origin
  • p53 Antibody (DO-1) is recommended for detection of wild type and mutant p53 under denaturing and non-denaturing conditions of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM
  • Anti-p53 Antibody (DO-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to biotin for WB, IHC(P) and ELISA; and to either TRITC or Alexa Fluor® 405 for IF, IHC(P) and FCM
  • TransCruz reagent for Gel Supershift and ChIP applications (sc-126 X, 200 µg/0.1 ml)
  • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of p53 (DO-1): sc-126.
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for p53 Antibody (DO-1) for WB and IHC(P) applications. These reagents are now offered in bundles with p53 Antibody (DO-1) (see ordering information below).

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p53 Antibody (DO-1) is an IgG2a κ mouse monoclonal p53 antibody (also designated tumor suppressor protein p53 antibody or TP53 antibody) that detects the p53 protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM. p53 Antibody (DO-1) is available as both the non-conjugated anti-p53 antibody form, as well as multiple conjugated forms of anti-p53 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. The 53 protein is a DNA-binding, oligomerization domain- and transcription activation domain-containing tumor suppressor. The p53 protein upregulates growth arrest and apoptosis-related genes in response to stress signals, thereby influencing programmed cell death, cell differentiation, and cell cycle control mechanisms. The p53 Antibody can stain both the nucleus and cytoplasm, as the p53 protein localizes to the nucleus, yet can be chaperoned to the cytoplasm by the negative regulator, MDM2. MDM2 is an E3 ubiquitin ligase that is upregulated in the presence of active p53, where it poly-ubiquitinates p53 for proteasome targeting. p53 fluctuates between latent and active DNA-binding conformations and is differentially activated through posttranslational modifications, including phosphorylation and acetylation. phospho-specific p53 Antibodies can differentiate between phosphorylated and non-phosphorylated p53. Mutations in the DNA-binding domain (DBD) of p53, amino acids 110-286, can compromise energetically-favorable association with cis elements and are implicated in several human cancers. p53 Antibody is therefore an important reagent for cancer research.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

References:

  1. p53 website and analysis of p53 gene mutations in human cancer: forging a link between epidemiology and carcinogenesis.  |  Soussi, T., et al. 2000. Hum Mutat. 15: 105-13. PMID: 10612830
  2. Regulation of p53 stability.  |  Ashcroft, M. and Vousden, KH. 1999. Oncogene. 18: 7637-43. PMID: 10618703
  3. The role of tetramerization in p53 function.  |  Chène, P. 2001. Oncogene. 20: 2611-7. PMID: 11420672
  4. Distinct pattern of p53 phosphorylation in human tumors.  |  Minamoto, T., et al. 2001. Oncogene. 20: 3341-7. PMID: 11423984
  5. Regulation of the specific DNA binding function of p53.  |  Hupp, TR., et al. 1992. Cell. 71: 875-86. PMID: 1423635
  6. Isolation of human-p53-specific monoclonal antibodies and their use in the studies of human p53 expression.  |  Banks, L., et al. 1986. Eur J Biochem. 159: 529-34. PMID: 2428616
  7. p53, the cellular gatekeeper for growth and division.  |  Levine, AJ. 1997. Cell. 88: 323-31. PMID: 9039259

How to dilute the sc-126 HRP antibody for WB? And for how long should the WB be incubated?

Asked by: llllllllllllll
Thank you for your question. Here is our recommended buffer. : https://www.scbt.com/p/tbs-blotto-a?requestFrom=search . Incubate the antibody overnight at 4 degrees. Here is our protocol : https://www.scbt.com/resources/protocols/western-immuno-blotting
Answered by: BlakeJ
Date published: 2024-02-02

ウエスタンブロットの実験に使用するにあたり、ネガティブコントロールとなる細胞株などはありますでしょうか？

Asked by: dm21030
質問頂き、誠にありがとうございます。下のウェブサイトをご参考ください。宜しくお願い致します。 https://www.proteinatlas.org/ENSG00000141510-TP53/cell+line Thank you very much for your question. Please refer to the website below. Thank you. https://www.proteinatlas.org/ENSG00000141510-TP53/cell Line
Answered by: Jiajun Z
Date published: 2023-09-05

What's the reactivity of the antibody? It can be used in mouse cell lines by WB? 

Asked by: fn89
Thank you for your question. p53 Antibody (DO-1) is recommended for detection of wild type and mutant p53 under denaturing and non-denaturing conditions of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM and can be used in western blotting experiments using murine cell lines.
Answered by: Tech Support Europe
Date published: 2023-08-28

Do you have an antibody that only detects the wild type p53 and not the mutant p53?

Asked by: SH_mountsinai
Thank you for your question . We do not have a p53 antibody that only detects the wild type.
Answered by: BlakeJ
Date published: 2023-07-12

Although very specific at ~ 53 kDa, the western blot shows two bands instead of one. What is the reason for that?

Asked by: TCP88
It seems there is an isoform which would be the reason for another band being shown. For a more detailed explanation please call our technical service line at 800-457-3801.
Answered by: Technical Service
Date published: 2023-04-26

What lots are available for sc-126 p53 (DO-1)

Asked by: Renny
Thank you for your question. For stock and quote requests please contact technical service by phone or email. [email protected] or (800)457-3801.
Answered by: BlakeJ
Date published: 2023-03-29

Did anyone used this antibody with only DBD and had signal? The epitope is at the N-terminal of the full protein, but we are getting dot blot signal with the DBD.

Asked by: DBD binding
Thank you for your question. Please contact our technical service team directly for troubleshooting and discussion of your experiment.
Answered by: Tech Support Europe
Date published: 2023-03-21

这个抗体适用于斑马鱼p53的免疫组化吗？

Asked by: cxfan
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2022-06-02
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Rated 4 out of 5 by virology99 from 使いやすい サンプルを頂き、とても使いやすかったので購入を検討しています。ノンスペもなくバンドをはっきり検出できます。
Date published: 2024-04-03
Rated 5 out of 5 by Cxlxy from well function in WB and IF Good results when tested p53 in western blot of whole cell extracts or IFfrom rat articular chondrocytes.
Date published: 2023-12-22
Rated 5 out of 5 by HL Yang lab from p53 antibody The antibody is very good. The band is clear on western blot.
Date published: 2023-08-14
Rated 5 out of 5 by danverg from Anti-p53 per analisi in western blot L'Ab è stato testato utilizzando un lisato cellulare di MDA-231 ottenuto in RIPA buffer. 20 ug di proteine sono state separate tramite SDS-Page e trasferite su un filtro di nitrocellulosa. Il bloccaggio è stato effettuato in Blotto A, l'Ab primario è stato utilizzato alla diluizione di 1:1000 in 10 mL di Blotto A. Il secondario 1:2000 in Blotto A. Il segnale ottenuto è pulito, con un'unica banda del perso molecolare atteso.
Date published: 2023-06-20
Rated 5 out of 5 by JLRosa from Detection of p53 in human cells We have tested the anti-p53 antibody (sc-126) from Santa Cruz Biotecnology by immunoblotting. We have obtained excellent results in human cells such as U2OS, HEK293T and A549.
Date published: 2023-05-16
Rated 5 out of 5 by Frazer Bell 1 from Equine sarcoid positive staining We tested this on equine sarcoids and staining was good using HIER with a EDTA pH9 buffer and a antibody dilution of 1:50.
Date published: 2023-04-21
Rated 5 out of 5 by kuanlab from western blot The antibody can gave a nice clean band. It is a good antibody.
Date published: 2023-03-10
Rated 5 out of 5 by vtn55 from Western blots The antibody provides clear signals without high background by western blots using several human cell lines.
Date published: 2022-12-05
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p53 Antibody (DO-1) is rated 4.6 out of 5 by 179.
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