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p53 Antibody (Pab 240): sc-99
- p53 Antibody (Pab 240) is a mouse monoclonal IgG1 κ, cited in 468 publications, provided at 200 µg/ml
- raised against amino acids 213-217 of p53 of human origin
- p53 Antibody (Pab 240) is recommended for detection of only mutant p53 under non-denaturing conditions but is equally reactive with mutant and wildtype p53 under denaturing conditions of mouse, rat, human and origin by WB, IP and IF; also reactive with additional species, including bovine and avian
- Anti-p53 Antibody (Pab 240) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of p53 (Pab 240): sc-99.
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for p53 Antibody (Pab 240) for WB applications. These reagents are now offered in bundles with p53 Antibody (Pab 240) (see ordering information below).
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p53 Antibody (Pab 240) is an IgG1 κ mouse monoclonal p53 antibody (also designated p53 antibody or TP53 antibody) that detects only mutant p53 under non-denaturing conditions but is equally reactive with mutant and wildtype p53 under denaturing conditions of mouse, rat and human origin by WB, IP and IF. p53 Antibody (Pab 240) is available as the non-conjugated anti-p53 antibody form, as well as multiple conjugated forms of anti-p53 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. p53, a DNA-binding, oligomerization domain- and transcription activation domain-containing tumor suppressor, upregulates growth arrest and apoptosis-related genes in response to stress signals, thereby influencing programmed cell death, cell differentiation, and cell cycle control mechanisms. p53 localizes to the nucleus, yet can be chaperoned to the cytoplasm by the negative regulator, MDM2. MDM2 is an E3 ubiquitin ligase that is upregulated in the presence of active p53, where it poly-ubiquitinates p53 for proteasome targeting. p53 fluctuates between latent and active DNA-binding conformations and is differentially activated through posttranslational modifications, including phosphorylation and acetylation. Mutations in the DNA-binding domain (DBD) of p53, amino acids 110-286, can compromise energetically-favorable association with cis elements and are implicated in several human cancers.
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