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p53 Antibody (Pab 1801): sc-98

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Datasheets
  • p53 Antibody (Pab 1801) is a mouse monoclonal IgG1 κ, cited in 407 publications, provided at 200 µg/ml
  • N-terminal epitope mapping between amino acids 32-79 of p53 of human origin
  • Anti-p53 Antibody (Pab 1801) is recommended for detection of p53 of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM
  • p53 Antibody (Pab 1801) is available conjugated to agarose for IP; and to phycoerythrin for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • TransCruz reagent for Gel Supershift and ChIP applications (sc-98 X, 200 µg/0.1 ml)
  • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of p53 (Pab 1801): sc-98.
  • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for p53 Antibody (Pab 1801) for WB and IHC(P) applications. These reagents are now offered in bundles with p53 Antibody (Pab 1801) (see ordering information below).

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p53 Antibody (Pab 1801) is an IgG1 κ mouse monoclonal p53 antibody (also designated p53 antibody or TP53 antibody) that detects p53 of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM. p53 Antibody (Pab 1801) is available as both conjugated and non-conjugated anti-p53 antibody forms. p53, a DNA-binding, oligomerization domain- and transcription activation domain-containing tumor suppressor, upregulates growth arrest and apoptosis-related genes in response to stress signals, thereby influencing programmed cell death, cell differentiation, and cell cycle control mechanisms. p53 localizes to the nucleus, yet can be chaperoned to the cytoplasm by the negative regulator, MDM2. MDM2 is an E3 ubiquitin ligase that is upregulated in the presence of active p53, where it poly-ubiquitinates p53 for proteasome targeting. p53 fluctuates between latent and active DNA-binding conformations and is differentially activated through posttranslational modifications, including phosphorylation and acetylation. Mutations in the DNA-binding domain (DBD) of p53, amino acids 110-286, can compromise energetically-favorable association with cis elements and are implicated in several human cancers.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

What are the differences of Anti-P53 Pab 1801,240and DO-1?If it possible to be used for mitochondria protein WB and staining?Which one is feasible?

Asked by: fwang
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-22

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue?

Asked by: DefinitelyNotMatt
Thank you for your question. Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is currently available conjugated to phycoerythrin (sc-98 PE), Alexa Fluor® 488 (sc-98 AF488) or Alexa Fluor® 647 (sc-98 AF647). Please contact Technical Service if you have further questions concerning this product.
Answered by: Tech Service 9
Date published: 2023-10-12
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Rated 4 out of 5 by karra from I recommend this antibody It was used in WB, SH-S5SY cells, and the dillution was 1/500. I totally recommend it
Date published: 2019-04-01
Rated 5 out of 5 by passion0510 from Great product I used it for immunihistochemistry and working great. I will recommend this antibody to others too.
Date published: 2018-03-22
Rated 1 out of 5 by Virge from IHC on mouse tissue not working I tried this antibody on immunoperoxidase staining of formalin-fixed, paraffin-embedded mouse breast carcinoma tissue at 1/50 and it did not work. No staining.
Date published: 2017-11-30
Rated 4 out of 5 by ladams2 from Worked well on primary cultures I received a sample of this p53 antibody because I was having difficulty detecting p53 in my primary murine astrocyte cultures by western blot. This gave a single, clear band, although I had to use a higher titer of antibody (1:100 dilution) to detect.
Date published: 2017-10-17
Rated 4 out of 5 by Tsen from Good antibody Worked well with western blotting at 1:500 dilution.
Date published: 2017-09-27
Rated 5 out of 5 by 小新贝贝 from Excellent! 1:250 diluted for wb of rat protein ,the band is strong with at the position of about 60KD.
Date published: 2017-08-08
Rated 5 out of 5 by mingerzhuan from exprected result It works very well for IF analysis of PFA fixed cells at a dilution of 1:50,the staining is our exprected result.
Date published: 2017-07-29
Rated 5 out of 5 by yujiao_ch from good IHC result Uses for formalin fixed tissue; IHC; routinely used over years, satisfied with results obtained.
Date published: 2017-05-27
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p53 Antibody (Pab 1801) is rated 4.6 out of 5 by 18.
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