NCAM/CD56 Antibody (ERIC 1): sc-106

NCAM/CD56 Antibody (ERIC 1) is an IgG1 κ mouse monoclonal NCAM antibody that detects NCAM of human origin by WB, IP, IF, IHC(P) and FCM. NCAM/CD56 Antibody (ERIC 1) is available as both conjugated and non-conjugated anti-NCAM antibody forms. Neural cell adhesion molecules (NCAMs) are a family of closely related cell surface glycoproteins involved in cell to cell interactions during growth and thought to play an important role in embryogenesis and development. The expression of these molecules is widespread in all three germ layers during embryogenesis, but is more restrictive in adult tissues. NCAM expression is observed in a variety of human tumors including neuroblastomas, rhabdo-myosarcomas, Wilms′ tumor, Ewing′s sarcoma and some primitive myeloid malignancies. Multiple isoforms of NCAM have been reported in both mouse and human brain tissue. In humans, NCAMs arise from differential splicing and use of alternative polyadenylation sites of a single gene mapping to 11q23.

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References:

1. Expression of neural cell adhesion molecule (NCAM) isoforms in neuroblastoma.  |  Phimister, E., et al. 1991. J Clin Pathol. 44: 580-5. PMID: 1856291
2. A monoclonal antibody (ERIC-1), raised against retinoblastoma, that recognizes the neural cell adhesion molecule (NCAM) expressed on brain and tumours arising from the neuroectoderm.  |  Bourne, SP., et al. 1991. J Neurooncol. 10: 111-9. PMID: 1895159
3. The N-cam gene is a complex transcriptional unit.  |  Walsh, FS. 1988. Neurochem Int. 12: 263-7. PMID: 20501228
4. Differential spectrum of expression of neural cell adhesion molecule isoforms and L1 adhesion molecules on human neuroectodermal tumors.  |  Figarella-Branger, DF., et al. 1990. Cancer Res. 50: 6364-70. PMID: 2400996
5. Presence of the long chain form of polysialic acid of the neural cell adhesion molecule in Wilms′ tumor. Identification of a cell adhesion molecule as an oncodevelopmental antigen and implications for tumor histogenesis.  |  Roth, J., et al. 1988. Am J Pathol. 133: 227-40. PMID: 2461089
6. Identity of Leu-19 (CD56) leukocyte differentiation antigen and neural cell adhesion molecule.  |  Lanier, LL., et al. 1989. J Exp Med. 169: 2233-8. PMID: 2471777
7. Characterization of neural cell adhesion molecules (NCAM) expressed by Ewing and neuroblastoma cell lines.  |  Lipinski, M., et al. 1987. Int J Cancer. 40: 81-6. PMID: 3036723
8. Neural cell adhesion molecule: structure, immunoglobulin-like domains, cell surface modulation, and alternative RNA splicing.  |  Cunningham, BA., et al. 1987. Science. 236: 799-806. PMID: 3576199
9. Cell adhesion and the molecular processes of morphogenesis.  |  Edelman, GM. 1985. Annu Rev Biochem. 54: 135-69. PMID: 3896119

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NCAM/CD56 Antibody (ERIC 1) has 64 citations in a variety of scientific publications.

Primary antibodies like Anti-NCAM/CD56 Antibody (ERIC 1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NCAM/CD56 Antibody (ERIC 1) Specifications

Alternate Product Name

CD56

Product Clone Name

ERIC 1

Classification

Primary Antibody

Clonality

Monoclonal Antibody

Antibody Isotype

IgG₁ kappa light chain

Amino Acids

Epitope Target

NCAM

Epitope Species

human

Product Reactivity/Detection

NCAM

Species Reactivity/Detection

human

Additional Mammalian Reactivity

N/A

Additional Non-Mammalian Reactivity

N/A

Applications

WB, IP, IF, IHC(P), FCM

Vialing Information

Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations

64 citations in various scientific publications

Recommended Secondary Detection Reagents

Mouse IgG Fc Binding Proteins

• m-IgG Fc BP-HRP: sc-525409 (Mouse IgG Fc binding protein conjugated to horseradish peroxidase)
• m-IgG Fc BP-B: sc-533652 (Mouse IgG Fc binding protein conjugated to biotin)
• m-IgG Fc BP-FITC: sc-533651 (Mouse IgG Fc binding protein conjugated to fluorescein isothiocyanate)
• m-IgG Fc BP-CFL 488: sc-533653 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 488)
• m-IgG Fc BP-CFL 555: sc-533654 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 555)
• m-IgG Fc BP-CFL 594: sc-533655 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 594)
• m-IgG Fc BP-CFL 647: sc-533656 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 647)
• m-IgG Fc BP-CFL 680: sc-533657 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 680)
• m-IgG Fc BP-CFL 790: sc-533658 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 790)

• m-IgG₁ BP-HRP: sc-525408 (Mouse IgG₁ binding protein conjugated to horseradish peroxidase)
• m-IgG₁ BP-B: sc-533660 (Mouse IgG₁ binding protein conjugated to biotin)
• m-IgG₁ BP-FITC: sc-533659 (Mouse IgG₁ binding protein conjugated to fluorescein isothiocyanate)
• m-IgG₁ BP-CFL 488: sc-533661 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 488)
• m-IgG₁ BP-CFL 555: sc-533662 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 555)
• m-IgG₁ BP-CFL 594: sc-533663 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 594)
• m-IgG₁ BP-CFL 647: sc-533664 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 647)
• m-IgG₁ BP-CFL 680: sc-533665 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 680)
• m-IgG₁ BP-CFL 790: sc-533666 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 790)

Available Conjugates/Forms

AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, B: Biotin, FITC: Fluoroscein, PE: Phycoerythrin