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SCBT

Anticorps CD35 (E-11): sc-7308

Fiches techniques
  • L'anticorps CD35 E-11 est un monoclonal IgG1 κ L'Anticorps CD35, cité dans 6 publications, fourni en 200 µg/ml
  • raised against intact human monocytes
  • L'Anticorps CD35 (E-11) est recommandé pour la détection de CD35 d'origine human par WB, IP, IF, IHC(P), FCM et ELISA
  • Anti-L'Anticorps CD35 (E-11) est disponible conjugué à l'agarose pour IP; à l'HRP pour WB, IHC(P) et ELISA; et soit à la phycoerythrin ou FITC pour IF, IHC(P) et FCM
  • aussi disponible conjugué à l'Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 ou Alexa Fluor® 647 pour WB (RGB), IF, IHC(P) et FCM
  • aussi disponible conjugué à l'Alexa Fluor® 680 ou Alexa Fluor® 790 pour WB (NIR), IF et FCM
  • Contactez notre Service Technique (ou votre Distributeur local) pour plus d'informations pour recevoir un échantillon GRATUIT de 10 µg de CD35 (E-11): sc-7308.
  • m-IgG Fc BP-HRP et m-IgG1 BP-HRP sont les réactifs secondaires recommandés pour la détection de l'anticorps CD35 (E-11) pour des applications de WB and IHC(P). Ces réactifs sont maintenant proposés en kit avec l'anticorps CD35 (E-11) (voir Informations pour la commande ci-dessous).

ACCÈS RAPIDE AUX LIENS

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CD35 Antibody (E-11) is an IgG1 κ mouse monoclonal CD35 antibody (also designated CR1 antibody, Complement C3b/C4b Receptor 1 (Knops Blood Group) antibody, Complement Receptor Type 1 antibody, C3b/C4b Receptor antibody, CD35 Antigen antibody, C3BR antibody, KN antibody, C3-Binding Protein antibody, or C4BR antibody) that detects the CD35 protein of human origin by WB, IP, IF, IHC(P), FCM and ELISA. CD35 Antibody (E-11) is available as both the non-conjugated anti-CD35 antibody form, as well as multiple conjugated forms of anti-CD35 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. CD35, also called complement receptor 1 (CR1), functions as the receptor for complement components C3b and C4b, and it mediates the phagocytosis by neutrophils and monocytes of particles coated with C3b or C4b. CD35 is expressed on granulocytes, monocytes, B cells, some NK cells and erythrocytes. CD35 is implicated in systemic lupus erythematosus (SLE), a chronic systemic autoimmune disease characterized by the production of a broad spectrum of autoantibodies against nuclear, cytoplasmic, and cell surface antigens and an overload of the immune complex. There is an increased proteolytic cleavage of leukocyte cell surface CD35 in SLE patients. Sequence analysis suggests that Crry may be the mouse genetic homolog of the CD35 antigen encoded by the human gene CR1.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

Can CD35 (E-11): sc-7308 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Posée par: TinTin
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, CD35 (E-11): sc-7308 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Répondue par: Technical Support
Date de publication: 2017-02-24
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Rated 3 de 5 de KeithJarosinski par Does not work on chicken HD11 cells I tested this antibody on chicken HD11 macrophage cell line and it did show positive staining using immunofluorescence assays. I also used the antibody on chicken DF-1 cells transfected with a chicken CD35 expression plasmid, but it also did not work in IFAs. I have not tested it in western blotting. It does not appear this antibody recognizes chicken CD35.
Date de publication: 2017-10-28
Rated 5 de 5 de xin wang par I have had enjoy experiences with santa's product When I was in school bought this antibody, I feel lucky
Date de publication: 2016-11-15
Rated 5 de 5 de HF par Produced beautiful immunoperoxidase membrane Produced beautiful immunoperoxidase membrane and cytoplasmic staining in formalin fixed, paraffin-embedded human kidney tissue. -SCBT QC
Date de publication: 2015-04-23
Rated 5 de 5 de QZ par Produced exceptional FCM data for human peripheral Produced exceptional FCM data for human peripheral blood leukocytes. -SCBT QC
Date de publication: 2013-10-19
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Anticorps CD35 (E-11) est évalué 4.5 de 5 de 4.
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