XPG Antibody (8H7): sc-13563

XPG Antibody (8H7) is an IgG_2a κ mouse monoclonal XPG antibody (also designated XPG antibody) that detects the XPG protein of human origin by WB, IP, IF and IHC(P). XPG Antibody (8H7) is available as both the non-conjugated anti-XPG antibody form, as well as multiple conjugated forms of anti-XPG antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor^® conjugates. Seven complementation groups (A-G) of xeroderma pigmentosum have been described. The xeroderma pigmentosum group A protein, XPA, is a zinc metalloprotein which preferentially binds to DNA damaged by ultraviolet (UV) radiation and chemical carcinogens. XPA is a DNA repair enzyme that has been shown to be required for the incision step of nucleotide excision repair. XPG (also designated ERCC5) is an endonuclease that makes the 3′ incision in DNA nucleotide excision repair. Mammalian XPG is similar in sequence to yeast RAD2. Conserved residues in the catalytic center of XPG are important for nuclease activity and function in nucleotide excision repair.

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References:

1. Conserved residues of human XPG protein important for nuclease activity and function in nucleotide excision repair.  |  Constantinou, A., et al. 1999. J Biol Chem. 274: 5637-48. PMID: 10026181
2. The relative expression of mutated XPB genes results in xeroderma pigmentosum/Cockayne′s syndrome or trichothiodystrophy cellular phenotypes.  |  Riou, L., et al. 1999. Hum Mol Genet. 8: 1125-33. PMID: 10332046
3. The XPV (xeroderma pigmentosum variant) gene encodes human DNA polymerase eta.  |  Masutani, C., et al. 1999. Nature. 399: 700-4. PMID: 10385124
4. An ERCC5 gene with homology to yeast RAD2 is involved in group G xeroderma pigmentosum.  |  Shiomi, T., et al. 1994. Mutat Res. 314: 167-75. PMID: 7510366
5. High incidence of ultraviolet-B-or chemical-carcinogen-induced skin tumours in mice lacking the xeroderma pigmentosum group A gene.  |  Nakane, H., et al. 1995. Nature. 377: 165-8. PMID: 7675085
6. Mutations in XPA that prevent association with ERCC1 are defective in nucleotide excision repair.  |  Li, L., et al. 1995. Mol Cell Biol. 15: 1993-8. PMID: 7891694
7. Complementation of the DNA repair defect in xeroderma pigmentosum group G cells by a human cDNA related to yeast RAD2.  |  Scherly, D., et al. 1993. Nature. 363: 182-5. PMID: 8483504
8. Identification of a damaged-DNA binding domain of the XPA protein.  |  Kuraoka, I., et al. 1996. Mutat Res. 362: 87-95. PMID: 8538652
9. Separation of protein factors that correct the defects in the seven complementation groups of xeroderma pigmentosum cells.  |  Tateishi, S., et al. 1995. J Biochem. 118: 819-24. PMID: 8576098
10. The DNA helicases acting in nucleotide excision repair, XPD, CSB and XPB, are not required for PCNA-dependent repair of abasic sites.  |  Cappelli, E., et al. 1999. Eur J Biochem. 259: 325-30. PMID: 9914510

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-XPG Antibody (8H7) has 23 citations in a variety of scientific publications.

Primary antibodies like Anti-XPG Antibody (8H7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

XPG Antibody (8H7) Specifications

Alternate Product Name

XPG

Product Clone Name

8H7

Classification

Primary Antibody

Clonality

Monoclonal Antibody

Antibody Isotype

IgG_2a kappa light chain

Amino Acids

Epitope Target

XPG

Epitope Species

human

Product Reactivity/Detection

XPG

Species Reactivity/Detection

human

Additional Mammalian Reactivity

N/A

Additional Non-Mammalian Reactivity

N/A

Applications

WB, IP, IF, IHC(P)

Vialing Information

Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations

23 citations in various scientific publications

Recommended Secondary Detection Reagents

Mouse IgG Fc Binding Proteins

• m-IgG Fc BP-HRP: sc-525409 (Mouse IgG Fc binding protein conjugated to horseradish peroxidase)
• m-IgG Fc BP-B: sc-533652 (Mouse IgG Fc binding protein conjugated to biotin)
• m-IgG Fc BP-FITC: sc-533651 (Mouse IgG Fc binding protein conjugated to fluorescein isothiocyanate)
• m-IgG Fc BP-CFL 488: sc-533653 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 488)
• m-IgG Fc BP-CFL 555: sc-533654 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 555)
• m-IgG Fc BP-CFL 594: sc-533655 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 594)
• m-IgG Fc BP-CFL 647: sc-533656 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 647)
• m-IgG Fc BP-CFL 680: sc-533657 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 680)
• m-IgG Fc BP-CFL 790: sc-533658 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 790)

• m-IgG_2a BP-HRP: sc-542731 (Mouse IgG_2a binding protein conjugated to horseradish peroxidase)
• m-IgG_2a BP-B: sc-542734 (Mouse IgG_2a binding protein conjugated to biotin)
• m-IgG_2a BP-FITC: sc-542733 (Mouse IgG_2a binding protein conjugated to fluorescein isothiocyanate)
• m-IgG_2a BP-CFL 488: sc-542735 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 488)
• m-IgG_2a BP-CFL 555: sc-542736 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 555)
• m-IgG_2a BP-CFL 594: sc-542737 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 594)
• m-IgG_2a BP-CFL 647: sc-542738 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 647)
• m-IgG_2a BP-CFL 680: sc-542739 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 680)
• m-IgG_2a BP-CFL 790: sc-542740 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 790)

• m-IgGκ BP-HRP: sc-516102 (Mouse IgGκ light chain binding protein conjugated to horseradish peroxidase)
• m-IgGκ BP-B: sc-516142 (Mouse IgGκ light chain binding protein conjugated to biotin)
• m-IgGκ BP-FITC: sc-516140 (Mouse IgGκ light chain binding protein conjugated to fluorescein isothiocyanate)
• m-IgGκ BP-PE: sc-516141 (Mouse IgGκ light chain binding protein conjugated to phycoerythrin)
• m-IgGκ BP-CFL 488: sc-516176 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 488)
• m-IgGκ BP-CFL 555: sc-516177 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 555)
• m-IgGκ BP-CFL 594: sc-516178 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 594)
• m-IgGκ BP-CFL 647: sc-516179 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 647)
• m-IgGκ BP-CFL 680: sc-516180 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 680)
• m-IgGκ BP-CFL 790: sc-516181 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 790)

Available Conjugates/Forms

AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin