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CD64 Antibody (10.1): sc-1184

Datasheets
  • CD64 Antibody (10.1) is a mouse monoclonal IgG1 κ CD64 antibody, cited in 21 publications, provided at 200 µg/ml
  • raised against human rheumatoid synovial fluid cells/monocytes
  • CD64 Antibody (10.1) is recommended for detection of CD64 cell surface marker of human origin by WB, IP, IF and FCM
  • Anti-CD64 Antibody (10.1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CD64 (10.1): sc-1184.
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CD64 Antibody (10.1) for WB applications. These reagents are now offered in bundles with CD64 Antibody (10.1) (see ordering information below).

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CD64 Antibody (10.1) is an IgG1 κ mouse monoclonal CD64 antibody (also designated FCGR1A antibody, FcγRI antibody, Fc gamma receptor Ia antibody, Fc fragment of IgG, high affinity Ia, receptor (CD64) antibody, high-affinity Fc-gamma receptor antibody, or integral membrane glycoprotein antibody) that detects the CD64 protein of human origin by WB, IP, IF and FCM. CD64 Antibody (10.1) is available as both the non-conjugated anti-CD64 antibody form, as well as multiple conjugated forms of anti-CD64 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Three different classes of IgG Fc receptors have been described: FcγRI (CD64), FcγRII (CD32) and FcγRIII (CD16). The low affinity receptors, CD64 and CD16, have a putative role in mediating humoral immune responses. CD64 is a surface glycoprotein with high affinity for monomeric IgG, is expressed constitutively on monocytes and macrophages, and can be induced in neutrophils subsequent to IFN-γ stimulation. CD64 plays a putative role in the initiation of cell-mediated cytotoxicity. Thus far, three genes encoding four distinct CD64 transcripts have been described. CD64 has been shown to associate with signal transducing subunit of the high affinity IgE receptor. Src family kinases Hck and Lyn show increased kinase activity and will co-immunoprecipitate with CD64 subsequent to receptor cross linking.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

References:

  1. Novel Fc gamma receptor I family gene products in human mononuclear cells.  |  Porges, AJ., et al. 1992. J Clin Invest. 90: 2102-9. PMID: 1430234
  2. Activation-dependent expression of low affinity IgG receptors Fc gamma RII(CD32) and Fc gamma RIII(CD16) in subpopulations of human T lymphocytes.  |  Engelhardt, W., et al. 1995. Immunobiology. 192: 297-320. PMID: 7649565
  3. IL-10 up-regulates human monocyte phagocytosis in the presence of IL-4 and IFN-gamma.  |  Capsoni, F., et al. 1995. J Leukoc Biol. 58: 351-8. PMID: 7665991
  4. Multiple regions of human Fc gamma RII (CD32) contribute to the binding of IgG.  |  Hulett, MD., et al. 1995. J Biol Chem. 270: 21188-94. PMID: 7673151
  5. Involvement of the high-affinity receptor for IgG (Fc gamma RI; CD64) in enhanced tumor cell cytotoxicity of neutrophils during granulocyte colony-stimulating factor therapy.  |  Valerius, T., et al. 1993. Blood. 82: 931-9. PMID: 7687898
  6. Physical and functional association of the high affinity immunoglobulin G receptor (Fc gamma RI) with the kinases Hck and Lyn.  |  Wang, AV., et al. 1994. J Exp Med. 180: 1165-70. PMID: 8064233

Esse anticorpo possui reatividade com CD64 de macaco cynomolgus?

Asked by: Marcelo Alves Pinto
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-23

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 7
Date published: 2017-02-09
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Rated 1 out of 5 by ifi15 from Doesn't work on FFPE sections The antibody doesn't work for IHC on FFPE. I’ve tried even tonsil and bone marrow. No reaction at all or nuclear staining if I concentrate the antibody.
Date published: 2021-09-24
Rated 4 out of 5 by Valeria92 from good antibody for IF I used this antibody in IF in frozen human colon tissue sections and it works very well.
Date published: 2018-06-20
Rated 5 out of 5 by FZC123 from Antibody working normally I used it (CD64 (10.1)) for detecting mouse liver by western blot (dilution 1:500),and appeared a strong bland.
Date published: 2017-07-05
Rated 5 out of 5 by Cweed from Great overall detection A great choice for CD64 detection. It stains and blots well and even works great for flow cytometry. This antibody is supported by more than ten citations.
Date published: 2017-01-20
Rated 4 out of 5 by vagabond123 from strong band at 50 kD There was a strong band at 50 kD in human decidual macrophage samples by WB
Date published: 2016-12-14
Rated 5 out of 5 by Bob S from Extremely satisfied WB data in human liver tissue. Extremely satisfied WB data were detected in human liver tissue! Cooooooool!
Date published: 2016-11-25
Rated 5 out of 5 by lihaiy from great product This product is very easy to use. I will buy it again next time.
Date published: 2016-11-01
Rated 5 out of 5 by CJ from 100 g/ml antibody used for IF with pfa fixed 100 g/ml antibody used for IF with pfa fixed Raji lymphoid cells. -SCBT Publication Review
Date published: 2015-07-02
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CD64 Antibody (10.1) is rated 4.3 out of 5 by 12.
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