Anti-Red Blood Cells antibody [34-3C] (ab106101)
Key features and details
- Mouse monoclonal [34-3C] to Red Blood Cells
- Suitable for: Flow Cyt, Functional Studies
- Reacts with: Mouse
- Isotype: IgG2a
Overview
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Product name
Anti-Red Blood Cells antibody [34-3C]
See all Red Blood Cells primary antibodies -
Description
Mouse monoclonal [34-3C] to Red Blood Cells -
Host species
Mouse -
Specificity
Recognizes an exposed surface determinant of intact Red Blood Cells. ab106101 only recognises antigenic determinants expressed on Mouse RBC and not on other species of RBC. -
Tested applications
Suitable for: Flow Cyt, Functional Studiesmore details -
Species reactivity
Reacts with: Mouse
Does not react with: Rat, Sheep, Rabbit, Chicken, Human -
Immunogen
Tissue, cells or virus corresponding to Mouse Red Blood Cells.
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Positive control
- Mouse erythrocytes
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General notes
This antibody efficiently binds to Fc receptors on macrophages inducing anemia in vivo due to a rapid Fc receptor (FcyR)-mediated erythrophagocytosis of opsonised RBC in spleen and livers. The capacity of the antibody to interact with FcyR is responsible for its haemolytic activity.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: 0.1% BSA, PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
0.2 µm filtered solution -
Primary antibody notes
This antibody efficiently binds to Fc receptors on macrophages inducing anemia in vivo due to a rapid Fc receptor (FcyR)-mediated erythrophagocytosis of opsonised RBC in spleen and livers. The capacity of the antibody to interact with FcyR is responsible for its haemolytic activity. -
Clonality
Monoclonal -
Clone number
34-3C -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab106101 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use at an assay dependent concentration.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
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Functional Studies |
Use at an assay dependent concentration.
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Notes |
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Flow Cyt
Use at an assay dependent concentration. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Functional Studies
Use at an assay dependent concentration. |
Target
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Relevance
Red blood cells are the most common type of blood cell and are the vertebrate body's principal means of delivering oxygen from the lungs or gills to body tissues via the blood. -
Alternative names
- Erythrocytes antibody
- RBCs antibody
Datasheets and documents
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Datasheet download
References (4)
ab106101 has been referenced in 4 publications.
- Akter J et al. Plasmodium-specific antibodies block in vivo parasite growth without clearing infected red blood cells. PLoS Pathog 15:e1007599 (2019). PubMed: 30811498
- Baudino L et al. Differential contribution of three activating IgG Fc receptors (FcgammaRI, FcgammaRIII, and FcgammaRIV) to IgG2a- and IgG2b-induced autoimmune hemolytic anemia in mice. J Immunol 180:1948-53 (2008). PubMed: 18209093
- Azeredo da Silveira S et al. Complement activation selectively potentiates the pathogenicity of the IgG2b and IgG3 isotypes of a high affinity anti-erythrocyte autoantibody. J Exp Med 195:665-72 (2002). PubMed: 11901193
- Fossati-Jimack L et al. High pathogenic potential of low-affinity autoantibodies in experimental autoimmune hemolytic anemia. J Exp Med 190:1689-96 (1999). PubMed: 10587359