Anti-gamma Actin antibody [2A3] (ab123034)
Key features and details
- Mouse monoclonal [2A3] to gamma Actin
- Suitable for: ELISA, IHC-Fr, WB, Flow Cyt, IHC-P, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Rabbit, Chicken, Human, Pig, Zebrafish
- Isotype: IgG2b
Overview
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Product name
Anti-gamma Actin antibody [2A3]
See all gamma Actin primary antibodies -
Description
Mouse monoclonal [2A3] to gamma Actin -
Host species
Mouse -
Specificity
This antibody does not cross react with other actin isoforms. The epitope recognized by ab123034 is highly conserved and the antibody is therefore expected to react with many other species.
This antibody is highly specific for gamma-cytoplasmic actin (γ-actin 1; encoded by ACTG1). It does not cross-react with the gamma-actin isoform expressed in the enteric smooth muscle tissues (γ-actin 2; encoded by ACTG2).
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Tested applications
Suitable for: ELISA, IHC-Fr, WB, Flow Cyt, IHC-P, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Rabbit, Chicken, Human, Pig, Zebrafish
Predicted to work with: a wide range of other species -
Immunogen
Synthetic peptide (Ac-
EEEIAALVI
-COOH) with an acetylated N-terminus and conjugated to KLH by a Cysteine residue linker, corresponding to N terminal amino acids 2-10 of Human gamma Actin -
Positive control
- ICC/IF: Human dermal fibroblast cells. IHC-P: Human small intestine and heart tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
2A3 -
Myeloma
NS0 -
Isotype
IgG2b -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab123034 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ELISA |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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WB | (2) |
Use at an assay dependent concentration. Predicted molecular weight: 42 kDa.
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Flow Cyt |
Use at an assay dependent concentration.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
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IHC-P | (2) |
1/100 - 1/500.
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IHC-P |
Use at an assay dependent concentration.
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ICC/IF |
1/500.
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Notes |
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ELISA
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 42 kDa. |
Flow Cyt
Use at an assay dependent concentration. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
IHC-P
1/100 - 1/500. |
IHC-P
Use at an assay dependent concentration. |
ICC/IF
1/500. |
Target
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Function
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. -
Involvement in disease
Defects in ACTG1 are the cause of deafness autosomal dominant type 20 (DFNA20) [MIM:604717]; also called autosomal dominant deafness type 26 (DFNA26). DFNA20 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. -
Sequence similarities
Belongs to the actin family. -
Post-translational
modificationsThe methylhistidine determined by Bienvenut et al is assumed to be the tele-methylhistidine isomer by similarity to the mouse ortholog. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 415296 Chicken
- Entrez Gene: 71 Human
- Entrez Gene: 11465 Mouse
- Entrez Gene: 100361457 Rat
- Entrez Gene: 287876 Rat
- Entrez Gene: 57935 Zebrafish
- Omim: 102560 Human
- SwissProt: Q5ZMQ2 Chicken
see all -
Alternative names
- ACT antibody
- ACTB antibody
- ACTG antibody
see all
Images
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Immunohistochemical analysis of paraffin embedded human heart tissue immunostained for gamma cytoplasmic actin using ab123034 at 1/100 dilution.
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Human dermal fibroblasts stained for gamma Actin (green) using ab123034 at 1/500 dilution in ICC/IF.
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Immunohistochemical analysis of paraffin embedded Human small intestine tissue labelled with Anti-gamma Actin antibody [2A3] at 5 μg/ml
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ab123034 (1:1000 dilution) stainign gamma Actin in the epithelial villi and smooth muscle cells in swine colon.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (16)
ab123034 has been referenced in 16 publications.
- Tang Y et al. Actin gamma 1 is a critical regulator of pancreatic ductal adenocarcinoma. Saudi J Gastroenterol 28:239-246 (2022). PubMed: 34856725
- Zhang L et al. Nujiangexanthone A Inhibits Hepatocellular Carcinoma Metastasis via Down Regulation of Cofilin 1. Front Cell Dev Biol 9:644716 (2021). PubMed: 33791303
- Lee LL et al. Cardiac pericytes function as key vasoactive cells to regulate homeostasis and disease. FEBS Open Bio 11:207-225 (2021). PubMed: 33135334
- Cui S et al. Transcriptional profiling of intervertebral disc in a post-traumatic early degeneration organ culture model. JOR Spine 4:e1146 (2021). PubMed: 34611583
- Yin C et al. Heat exposure impairs porcine oocyte quality with suppressed actin expression in cumulus cells and disrupted F-actin formation in transzonal projections. J Anim Sci Biotechnol 11:71 (2020). PubMed: 32647569