Anti-CD3 antibody [UCH-T1] (ab215267)
Key features and details
- Mouse monoclonal [UCH-T1] to CD3
- Suitable for: WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CD3 antibody [UCH-T1]
See all CD3 primary antibodies -
Description
Mouse monoclonal [UCH-T1] to CD3 -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human CD3. (Human infant thymocytes and Sezary cells).
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Positive control
- Molt4 and Jurkat whole cell lysate (ab7899); Human PBMCs.
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General notes
Clone UCH-T1 is considered a pan T-cell marker - it can be used for the detection of T cell populations in peripheral blood and lymph nodes and the categorisation of T versus B cell lymphomas and leukaemias. It reacts with the majority of peripheral blood T lymphocytes, a major proportion of thymocytes, the majority of T cell chronic lymphocytic leukaemia cells, Sézary leukaemias and approximately 70% of acute lymphoblastic leukaemias of T cell origin.
ab215267 is a recombinant monoclonal antibody produced from the original monoclonal with variable regions (i.e. specificity) from the hybridoma.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
UCH-T1 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab215267 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 3 - 4 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 19 kDa).
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Flow Cyt |
Use at an assay dependent concentration.
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Notes |
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WB
Use a concentration of 3 - 4 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 19 kDa). |
Flow Cyt
Use at an assay dependent concentration. |
Images
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All lanes : Anti-CD3 antibody [UCH-T1] (ab215267) at 3 µg/ml
Lane 1 : Jurkat cell lysate (in RIPA buffer)
Lane 2 : Molt4 cell lysate (in RIPA buffer)
Lysates/proteins at 35 µg per lane.
Predicted band size: 19 kDa
Observed band size: 23 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour.
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Flow cytometric analysis of the dose-dependency (D, F, H) of ab215267 binding to live human PBMCs (B). The gating strategy is shown for control puposes (A, B, C, E, G).
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab215267 has not yet been referenced specifically in any publications.