Anti-PHD1/prolyl hydroxylase antibody [PHD112/G7] (ab82884)
Key features and details
- Mouse monoclonal [PHD112/G7] to PHD1/prolyl hydroxylase
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgM
Overview
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Product name
Anti-PHD1/prolyl hydroxylase antibody [PHD112/G7]
See all PHD1/prolyl hydroxylase primary antibodies -
Description
Mouse monoclonal [PHD112/G7] to PHD1/prolyl hydroxylase -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length Human protein
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Positive control
- Human testis tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
PHD112/G7 -
Isotype
IgM -
Research areas
Associated products
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Compatible Secondaries
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab82884 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration.
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Notes |
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IHC-P
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
Target
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Function
Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. Functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. May play a role in cell growth regulation. Isoform p40 and isoform p43 exhibit the same level of activity. -
Tissue specificity
Expressed abundantly in all tissues with highest expression in testis. Expressed in hormone responsive tissues, including normal and cancerous mammary, ovarian and prostate epithelium. -
Sequence similarities
Contains 1 Fe2OG dioxygenase domain. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 112398 Human
- Omim: 606424 Human
- SwissProt: Q96KS0 Human
- Unigene: 515417 Human
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Alternative names
- DKFZp434E026 antibody
- EGL nine (C.elegans) homolog 2 antibody
- Egl nine homolog 2 (C. elegans) antibody
see all
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab82884 has been referenced in 1 publication.
- Andersen S et al. Overexpression of the HIF Hydroxylases PHD1, PHD2, PHD3 and FIH Are Individually and Collectively Unfavorable Prognosticators for NSCLC Survival. PLoS One 6:e23847 (2011). IHC-P ; Human . PubMed: 21887331