Anti-Hairy antibody [1/24] (ab20165)
Key features and details
- Mouse monoclonal [1/24] to Hairy
- Suitable for: ChIP, IHC, WB
- Reacts with: Drosophila melanogaster
- Isotype: IgG1
Overview
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Product name
Anti-Hairy antibody [1/24] -
Description
Mouse monoclonal [1/24] to Hairy -
Host species
Mouse -
Tested applications
Suitable for: ChIP, IHC, WBmore details -
Species reactivity
Reacts with: Drosophila melanogaster -
Immunogen
Other Immunogen Type corresponding to Hairy.
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General notes
This antibody stains Drosophila embryos.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading...
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Purity
Protein A/G purified -
Primary antibody notes
This antibody stains Drosophila embryos -
Clonality
Monoclonal -
Clone number
1/24 -
Myeloma
Sp2/0-Ag14 -
Isotype
IgG1 -
Light chain type
unknown -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab20165 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ChIP |
Use at an assay dependent concentration.
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IHC |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 37 kDa.
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Notes |
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ChIP
Use at an assay dependent concentration. |
IHC
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 37 kDa. |
Target
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Relevance
Pair rule protein that regulates embryonic segmentation and adult bristle patterning. Transcriptional repressor of genes that require a bHLH protein for their transcription (e.g. the Fushi tarazu gene. Transcription repression requires formation of a complex with a corepressor -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 38995 Drosophila melanogaster
- SwissProt: Q95NH3 Drosophila melanogaster
- SwissProt: Q95NU9 Drosophila melanogaster
- SwissProt: Q960W5 Drosophila melanogaster
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Alternative names
- hairy protein antibody
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab20165 has been referenced in 3 publications.
- Slaninova V et al. Notch stimulates growth by direct regulation of genes involved in the control of glycolysis and the tricarboxylic acid cycle. Open Biol 6:150155 (2016). Drosophila melanogaster . PubMed: 26887408
- Zhou D et al. Mechanisms Underlying Hypoxia Tolerance in Drosophila melanogaster: hairy as a metabolic switch. PLoS Genet 4:e1000221 (2008). Drosophila melanogaster . PubMed: 18927626
- Wainwright SM & Ish-Horowicz D Point mutations in the Drosophila hairy gene demonstrate in vivo requirements for basic, helix-loop-helix, and WRPW domains. Mol Cell Biol 12:2475-83 (1992). PubMed: 1588951