B6.Cg-Pik3c3tm1Bvan/H
| Status | Available to order |
| EMMA ID | EM:13251 |
| International strain name | B6.Cg-Pik3c3tm1Bvan/H |
| Alternative name | Vps34-flox |
| Strain type | Targeted Mutant Strains : Conditional mutation |
| Allele/Transgene symbol | Pik3c3tm1Bvan, |
| Gene/Transgene symbol | Pik3c3 |
Information from provider
| Provider | Bart Vanhaesebroeck |
| Provider affiliation | University College London |
| Genetic information | Exon 21 of the kinase domain of the Pik3c3 (Vps34) gene was flanked by loxP sites. Excision of exon 21 encoding the kinase domain of Vps34 specifically in the megakaryocyte/platelet lineage was obtained by breeding Pik3c3 lox/lox mice with Pf4-cre recombinase transgenic mice to generate Pf4-Cre-Pik3c3 lox/lox mice (referred to as Vps34-flox). |
| Phenotypic information | Homozygous:Mice are phenotypically normal.Heterozygous:Mice are phenotypically normal. |
| Breeding history | Backcrossed onto C57BL/6J for more than 10 generations. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
Literature references
- Vps34/PI3KC3 deletion in kidney proximal tubules impairs apical trafficking and blocks autophagic flux, causing a Fanconi-like syndrome and renal insufficiency.;Grieco Giuseppina, Janssens Virginie, Gaide Chevronnay Héloïse P, N'Kuli Francisca, Van Der Smissen Patrick, Wang Tongsong, Shan Jingdong, Vainio Seppo, Bilanges Benoit, Jouret François, Vanhaesebroeck Bart, Pierreux Christophe E, Courtoy Pierre J, ;2018;Scientific reports;8;14133; 30237523
- A dual role for the class III PI3K, Vps34, in platelet production and thrombus growth.;Valet Colin, Levade Marie, Chicanne Gaëtan, Bilanges Benoit, Cabou Cendrine, Viaud Julien, Gratacap Marie-Pierre, Gaits-Iacovoni Frédérique, Vanhaesebroeck Bart, Payrastre Bernard, Severin Sonia, ;2017;Blood;130;2032-2042; 28903944