B6;129P2-Hprttm1(CAG-EGFP)Laumm/H
| Status | Available to order |
| EMMA ID | EM:12427 |
| International strain name | B6;129P2-Hprttm1(CAG-EGFP)Laumm/H |
| Alternative name | LifeAct-GFP |
| Strain type | Targeted Mutant Strains : Conditional mutation |
| Allele/Transgene symbol | Hprttm1(CAG-EGFP)Laumm, |
| Gene/Transgene symbol | Hprt1 |
Information from provider
| Provider | Laura Machesky |
| Provider affiliation | The Beatson Institute for Cancer Research |
| Additional owner | LifecAct plasmid from Ibidi GFP patent owned by GE Healthcare, due to expire in Sept 2021 |
| Genetic information | The cre recombinase-inducible transgenic mice can conditionally express cell-type specific LifeAct-GFP. This is possible due to a floxed neomycin resistance 'STOP' cassette encoded upstream of the LifeAct-GFP fusion cDNA in the Hprt locus of the mouse X chromosome. |
| Phenotypic information | Homozygous:No overt phenotype. LifeAct is a 17 amino acid peptide, isolated from yeast, which facilitates visualisation of filamentous actin (F-actin) in cells and tissues without significantly interfering with actin dynamics. This enables live imaging of the actin cytoskeletonHeterozygous:No overt phenotype. LifeAct is a 17 amino acid peptide, isolated from yeast, which facilitates visualisation of filamentous actin (F-actin) in cells and tissues without significantly interfering with actin dynamics. This enables live imaging of the actin cytoskeleton |
| Breeding history | Intercrossing of LifeAct-GFP mice yielded the expected Mendelian ratios, indicating that no embryonic lethality had occurred. Moreover, LifeAct-GFP male and female mice are fertile and have no major defects in any organs studied. Animals are reproductively mature at 8 weeks of age and a decline in reproductivity can be observed from 6 months of age. Average length of gestation is 19 days. |
| References |
|
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
Literature references
- Tissue inducible Lifeact expression allows visualization of actin dynamics in vivo and ex vivo.;Schachtner Hannah, Li Ang, Stevenson David, Calaminus Simon D J, Thomas Steve, Watson Steve P, Sixt Michael, Wedlich-Soldner Roland, Strathdee Douglas, Machesky Laura M, ;2012;European journal of cell biology;91;923-929; 22658956