Product Details
Alternative Name: | CCT, p63, Tric, T-complex 1 |
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Clone: | 23c |
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Host: | Rat |
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Isotype: | IgG2c |
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Immunogen: | Recombinant mouse TCP-1α (C-terminal fragment). |
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UniProt ID: | P11983 |
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GenBank ID: | D90344 |
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Source: | Purified from mouse ascites. |
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Species reactivity: | Human, Mouse, Rat Chicken, Dog, Hamster, Plant, Rabbit, Sheep
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Applications: | ICC, IHC, IP, WB
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Recommended Dilutions/Conditions: | Western Blot (1:1,000, colorimetric) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Application Notes: | Detects a band of ~60kDa by Western blot. |
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Purity Detail: | Protein G affinity purified. |
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Formulation: | Liquid. In PBS containing 50% glycerol and 0.09% sodium azide. |
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Handling: | Avoid freeze/thaw cycles. |
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Shipping: | Blue Ice |
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Long Term Storage: | -20°C |
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Scientific Background: | TCP-1alpha is a 60 kDa subunit of a cytosolic hetero-oligomer chaperone that is known to be involved in the folding of Actin and Tubulin. This protein is a member of the Chaperonin family, which includes E. coli GroEL, the mitochondrial Heat-shock protein Hsp60, the plastid Rubisco-subunit-binding protein, and the archaebacterial protein TF55. These Chaperonins assist the folding of proteins upon ATP hydrolysis. |
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Regulatory Status: | RUO - Research Use Only |
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Western blot analysis of TCP-1a: Lane 1: MW marker, Lane 2: Hsp60 Recombinant Human Protein, Lane 3: 3T3 Heat Shocked, Lane 4: PC-12 Heat Shocked.
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Product Literature References
Association of Simian Virus 40 Vp1 with 70-Kilodalton Heat ShockProteins and Viral Tumor Antigens: H. Kasamatsu, et al. ; J. Virol.
83, 37 (2008),
Application(s): WB, IP using monkey cell lysates,
Abstract;
Identification of a host protein essential for assembly of immature HIV-1 capsids: J.R. Lingappa, et al. ; Nature
415, 88 (2002),
Application(s): IP using plant (wheat) samples,
Abstract;
The interaction of the chaperonin tailless complex polypeptide 1 (TCP1) ring complex (TRiC) with ribosome-bound nascent chains examined using photo-cross-linking: J. Frydman, et al. ; J. Cell Biol.
149, 591 (2000),
Application(s): IP using rabbit samples,
Abstract;
A 102 kDa subunit of a Golgi-associated particle has homology to beta subunits of trimeric G proteins: K.R. Willison, et al. ; EMBO J.
12, 2847 (1993),
Application(s): WB, ICC using human, mouse, rat, & chicken samples,
Abstract;
T-complex polypeptide-1 is a subunit of a heteromeric particle in the eukaryotic cytosol: K. Willison, et al. ; Nature
358, 249 (1992),
Application(s): ICC using human samples,
Abstract;
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