Cat. #160885
Serum Free-Suspension adapted recombinant CHO-K1 cell line with integrated Technology for enhanced production of Human Erythropoietin protein.
Cat. #: 160885
Organism: Hamster
Tissue: Ovary
Disease: Therapeutics
£575.00
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Contributor
Inventor: Saiyed Taslimarif ; Patel Paresh Brijalal ; Mukhopadhyaya Rabindranath
Institute: The Centre for Cellular and Molecular Platforms C-CAMP
Tool Details
*FOR RESEARCH USE ONLY
- Name: Serum Free-Suspension adapted recombinant CHO-K1 cell line with integrated Technology for enhanced production of Human Erythropoietin protein.
- Alternate name: haematopoietin, or haemopoietin
- Parental cell: CHO-K1
- Organism: Hamster
- Tissue: Ovary
- Donor: The CHO-K1 cells were transduced with the two different lentiviral vectors for stable constitutive expression of 1) Human EPO gene and 2) a ShRNA targeting a specific protease that can cleave the EPO protein. G418 and Puromycin were used for selection of stable clones. The EPO is expressed without any affinity tag and is actively secreted in the culture supernatant. The adherent cells were adapted in serum free media (CD Forti media) and grown in shake flask.
- Disease: Therapeutics
- Conditional: No
- Production details: The CHO-K1 cells were transduced with the two different lentiviral vectors for stable constitutive expression of 1) Human EPO gene and 2) a ShRNA targeting a specific protease that can cleave the EPO protein. G418 and Puromycin were used for selection of stable clones. The EPO is expressed without any affinity tag and is actively secreted in the culture supernatant. The adherent cells were adapted in serum free media (CD Forti media) and grown in shake flask.
Target Details
- Target: Erythropoietin (EPO)
Handling
- Growth medium: Grown in Serum Free CD Forti media. The cells are grown in a CO2 incubator at 37?°C with 5% CO2 with shaking.
- Temperature: 37° C
- Atmosphere: 5% CO2
References
- A VECTOR, A HOST CELL AND A METHOD THEREOF (PCT/IB2019/052213)
