Cat. #160840
HeLa Flp-In SLC7A5+SLC7A5 3’UTR
Cat. #: 160840
Unit size: 1x10^6 cells / vial
Model: Knock-Out
£575.00
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Contributor
Inventor: Matthias Hentze
Institute: European Molecular Biology Laboratory (EMBL)
Tool Details
*FOR RESEARCH USE ONLY
- Name: HeLa Flp-In SLC7A5+SLC7A5 3’UTR
- Parental cell: HeLa FLP-IN T-REx
- Model: Knock-Out
- Description: To identify genes whose expression is controlled by YBX3
- Application: YBX3
- Production details: Adherent HeLa cells were grown at 37ÄÂ?°C and 5% CO2 in DMEM, supplemented with 10% FBS Gold (PAA), 1% Penicillin/Streptomycin (Sigma) and 1% L-glutamine (GIBCO). Cells were grown until 70%ÄË?Â?Â80% confluent, trypsinized, counted (Biorad TC20) and 200,000 cells were seeded per well in a 6-well dish for reverse transfection of siRNAs following the manufacturerÄË?Â?Âs recommendations (Lipofectamine RNAiMax, Invitrogen, cat# 137780755). YBX3 and control siRNAs were obtained from...
Target Details
- Target: YBX3
Applications
- Application: YBX3
Handling
- Format: Frozen
- Growth medium: Adherent HeLa cells were grown at 37?°C and 5% CO2 in DMEM, supplemented with 10% FBS Gold (PAA), 1% Penicillin/Streptomycin (Sigma) and 1% L-glutamine (GIBCO) Grown in medium containing blasticidine (5mg/ml) and hygromycin (200mg/ml). Cells were grown until 70%ÄË?Â?Â80% confluent, trypsinized, counted (Biorad TC20) and 200,000 cells were seeded per well in a 6-well dish for reverse transfection of siRNAs following the manufacturerÄË?Â?Âs recommendations (Lipofectamine RNAiMax,...
- Unit size: 1x10^6 cells / vial
Related Tools
- Related tools: HeLa Flp-In YBX3: GFP ; HeLa Flp-In SLC7A5 ; HeLa Flp-In SLC25A6 ; HeLa Flp-In SLC25A6+SLC7A5 3ÄË?Â? UTR
References
- The RNA-Binding Protein YBX3 Controls Amino Acid Levels by Regulating SLC mRNA Abundance
