Cat. #160725
Xist-TetOP mutant: DeltaB+1/2C cell line
Cat. #: 160725
Sub-type: Continuous
Unit size: 1x10^6 cells / vial
Organism: Mouse
Model: Stem Cells
£575.00
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Contributor
Inventor: Sim?Âo Teixeira da Rocha
Institute: Instituto de Medicina Molecular Jo?Âo Lobo Antunes
Tool Details
*FOR RESEARCH USE ONLY
- Name: Xist-TetOP mutant: DeltaB+1/2C cell line
- Tool sub type: Continuous
- Parental cell: Xist-TetOP XY ESCs (Wutz et al., 2002 PMID: 11780141)
- Organism: Mouse
- Growth properties: Disruption of Polycomb recruitment to the inactive X-chromosome.
- Model: Stem Cells
- Conditional: No
- Conditional description: Inducible through addition of doxycycline (1.5 ÄÂÄ?g/ml) to the medium
- Description: Deletion of mouse Xist lncRNA to dissect specific RNA modules in the process of X-chromosome inactivation.
- Production details: CRISPR/Cas9 genome editing of the Xist-TetOP XY ESC line
- Additional notes: Cancer Research Technology Limited (trading research tools as Ximbio) has been granted a non-exclusive license to the CRISPR-Cas9 technology by ERS Genomics Ltd under the patent rights listed here. This license from ERS Genomics Ltd allows Ximbio to develop and commercialise CRISPR-Cas9 modified cell lines for research use only. Ximbio can provide these modified CRISPR-Ca...
- Recommended controls: Xist-TetOP XY ESCs (Wutz et al., 2002 PMID: 11780141)
Target Details
- Target: Mouse Xist lncRNA
Handling
- Format: Frozen
- Growth medium: ESCs grow in feeder-free classic ESC medium DMEM media containing 15% fetal bovine serum (FBS), 103 U/ml leukemia inhibitor factor (LIF), 10-4 mM 2-mercaptoethanol, 50 U/ml penicillin, and 50 ?g/ml of streptomycin (Gibco) at 37?°C in 5-8% CO2. Medium is changed daily Inducible expression of Xist driven by a TetO promoter is achieved by adding DOX (1.5 ?g/ml).
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
Related Tools
- Related tools: Xist-TetOP mutant: ?B cell line ; Xist-TetOP mutant: ?C cell line
References
- Bousard et al. 2019. EMBO Rep. 20(10):e48019. PMID: 31456285.
