Cat. #160714
CD6-GFP Jurkat cell line
Cat. #: 160714
Sub-type: Continuous
Unit size: 1x10^6 cells / vial
Organism: Human
Tissue: Blood
Disease: Cancer
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Alessandra Cambi
Institute: Radboud University Medical Center
Tool Details
*FOR RESEARCH USE ONLY
- Name: CD6-GFP Jurkat cell line
- Alternate name: CD6
- Tool sub type: Continuous
- Parental cell: Jurkat E6.1
- Organism: Human
- Tissue: Blood
- Disease: Cancer
- Conditional: No
- Description: Activation of the T cell receptor (TCR) on the T cell through ligation with antigen-MHC complex of an antigen-presenting cell (APC) is an essential process in the activation of T cells and induction of the subsequent adaptive immune response. Upon activation, the TCR, together with its associated co-receptor CD3 complex, assembles in signaling microclusters that are transported to the center of the organizational structure at the T cell-APC interface termed the immunological synapse (IS). During IS formation, local cell surface receptors and associated intracellular molecules are reorganized, ultimately creating the typical bull's eye-shaped pattern of the IS. CD6 is a surface glycoprotein receptor, which has been previously shown to associate with CD3 and co-localize to the center of the IS in static conditions or stable T cell-APC contacts.
- Production details: Jurkat cell lines stably expressing CD6-GFP were obtained by electroporation using the Neon Transfection System for Electroporation (Invitrogen) according to the manufacturer's guidelines. Shortly, 5*105 Jurkat cells were transfected at 1325 Volt (10 ms, 3 pulses) with 3 ÄÂÄ?g of DNA in 100 ÄÂÄ?l Resuspension buffer. After transfection cells were seeded in 2 ml of 1640 RPMI with 10% FCS and 1% U-glut. Antibiotics were added after 3 h. Stable cell lines were sorted on GFP expression on...
Target Details
- Target: Cluster of Differentiation 6 labelled with GFP, CD6-GFP
Handling
- Format: Frozen
- Growth medium: 1640 RPMI supplemented with 10% Fetal Calf Serum, 1 mM Ultra-glutamine and antibiotics (100 U/ml penicillin, 100 ?g/ml streptomycin and 0.25 ?g/ml amphotericin B. 500 ng/ml geneticin.
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
Related Tools
- Related tools: CD6-RFP Jurkat cell line cell line ; CD6-LifeAct-GFP Jurkat cell line cell line
References
- Meddens et al. 2018. Front Immunol. 9:2333. PMID: 30356797.
