Cat. #160551
Luciferase-expressing D1-MSC cell line
Cat. #: 160551
Unit size: 1x10^6 cells / vial
Organism: Mouse
Tissue: Bone Marrow
Model: Stem Cells
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Jose Luis Pedraz ; Laura Saenz del Burgo ; JesÄ?s Ciriza
Institute: University of the Basque Country (EHU)
Tool Details
*FOR RESEARCH USE ONLY
- Name: Luciferase-expressing D1-MSC cell line
- Parental cell: D1-MSC
- Organism: Mouse
- Tissue: Bone Marrow
- Growth properties: Adherent cells
- Model: Stem Cells
- Conditional: No
- Description: Mesenchymal stem cells (MSCs) are multipotent stem cells found in bone marrow that are important for making and repairing skeletal tissues, such as cartilage, bone and the fat found in bone marrow. D1-MSC's are a cell line of murine MSCs. Luciferase is a generic term for the class of oxidative enzymes that produce bioluminescence, and is usually distinguished from a photoprotein. Luciferases are widely used in biotechnology, for microscopy and as reporter genes, for many of the same applications as fluorescent proteins. However, unlike fluorescent proteins, luciferases do not require an external light source, but do require addition of luciferin, the consumable substrate.The detection of luciferase can be used to track the location of MSCs by non invasive image techniques.
- Production details: pSIN-EF2-Luc-Pur was transfected into One Shot1 Stbl3TM Chemically Competent E. coli, extracted with QIAprep1 Spin Maxiprep Kit (Qiagen) and quantified with Nanodrop ND-1000 Spectrophotometer. 2.5 x106 293T cells were cultured with DMEM, 10% FCS and 1% P/S overnight and further transfected with 9mg of pSIN-EF2-Luc-Pur 3mg of pMD2.G (Addgene plasmid 12259), 6mg of psPAX2 (Addgene plasmid 12260) and 45mL of lipofectamine1 2000 in OptiMEM medium following providerÄË?Â?Âs recommendations. A...
- Recommended controls: WT D1-MSC
Target Details
- Target: Luciferase
Handling
- Format: Frozen
- Growth medium: DMEM supplemented with 10% FCS and P/S
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
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