Cat. #159695
Immorta-MAIT T cell clone IK-C1 cell line
Cat. #: 159695
Unit size: 1x10^6 cells / vial
Organism: Human
Disease: Mycobacterium tuberculosis
Model: Immortalised Line
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Marielle Gold ; Erin Meerseier ; Irina Kurtz ; David Lewinsohn
Institute: Oregon Health & Science University (OHSU)
Tool Details
*FOR RESEARCH USE ONLY
- Name: Immorta-MAIT T cell clone IK-C1 cell line
- Organism: Human
- Disease: Mycobacterium tuberculosis
- Growth properties: TRAV1-2 is expressed uniformly (determined by flow cytometry staining). This clone expresses CD4, CD8, CD161, and CD27 and can be classified as a double-positive thymocyte. This T cell clone binds the MR1/5-OP-RU tetramer but not MR1/6FP tetramer (negative control). Clone is MR1-restricted in its production of IFN-gamma by ELISPOT test, determined by its response to M. smegmatis-infected A549 cell line but not a M. smegmatis-infected MR1-- A549 cell line.
- Model: Immortalised Line
- Conditional: No
- Description: Mucosal-associated invarient T (MAIT) cells are innate-like T cells (a subset of T cells) that are found in blood, liver, lungs, and mucosa and are known to play a role in defense against bacterial and viral infections. MAITs have also been shown to potentially play a role in autoimmune diseases such as multiple sclerosis, rheumatiod arthritis, and systemic lupis erythematosus.
- Production details: Generated by single cell isolation of MR1/5-OP-RU tetramer+ cells from a human thymus. Single cells were rapidly expanded into a T cell clone using antibody to CD3 (clone OKT3) and IL-2.
Handling
- Format: Frozen
- Growth medium: Can be use with conventional T cell expansion methods and proliferates under simple culture methods. Cell line can be maintained for at least 3 months. Upon receival spin cell and resuspend them in 15ml of fresh media and add to T12.5 flask. Media used was RPMI, supplemented with, 10% FBS, L-Glut, gentamicin and 0.5ng/mL IL2. Incubate at 37 degrees C. Aspirate half the media and replace with fresh media every 3-4 weeks.
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice