#157660

DLKP-M cell line

Cat. #157660

DLKP-M cell line

Cat. #: 157660

Sub-type: Continuous

Unit size: 1x10^6 cells / vial

Availability: 3-4 weeks

Organism: Human

Tissue: Lung

Disease: Cancer

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Martin Clynes

Institute: Dublin City University

Tool Details
Target Details
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: DLKP-M cell line
  • Tool sub type: Continuous
  • Parental cell: DLKP
  • Organism: Human
  • Tissue: Lung
  • Disease: Cancer
  • Growth properties: Monolayer
  • Description: Mesenchymal-like sub-population cloned at NCTCC from DLKP P5, about 5% of DLKP cells show DLKP-M morphology, interconversion with -I, closest to normal chromosome number, fold resistance comp. to DLKP: 0.8 Adr; 0.95 Vcr; 0.7 VP-16. Immunocytochemistry: cytokeratin (all)-; GFAP-vimentin+; neurofilament+; P-gp-; a2- integrin++; 703D4+; 704A1 +; desmin-. More invasive in vitro than DLKP-SQ.
  • Production details: Single cell cloning from DLKP parental population
  • Biosafety level: 1
  • Recommended controls: DLKP parental line
  • Cellosaurus id: CVCL_H739

Target Details

  • Target: DLKP

Handling

  • Format: Frozen
  • Growth medium: DMEM/Ham's F12, 5% FCS. Split 1:5, medium change 2-3 times weekly. Freezing method: 10% DMSO in FBS
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice
  • Storage conditions: Liquid Nitrogen
  • Mycoplasma free: Yes

References

  • O'Sullivan et al. 2014. Tumour Biol. 35(7):6983-97. PMID: 24744142.
  • Keenan et al. 2012. Exp Cell Res. 318(5):593-602. PMID: 22281030.
  • McBride et al. 1998. Tumour Biol. 19(2):88-103. PMID: 9486560.