Cat. #157646
NF70 peptide
Cat. #: 157646
Sub-type: Synthetic Peptide
Availability: Please enquire for quantities and pricing
Target: Other
Function: siRNA delivery
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Piret Arukuusk ; Ly Porosk ; Ülo Langel
Institute: University of Tartu
Tool Details
*FOR RESEARCH USE ONLY
- Tool name: NF70 peptide
- Alternate name: NickFect7
- Tool sub type: Synthetic Peptide
- Sequence: HHHHYHHGO(ILLKALKALAKAIL)
- Cellular tissue localisation: Cell-penetrating peptide to assist delivery of siRNA into cells via endocytosis
- Source: Solid-phase synthesis
- Target: Other
- Description: This peptide can be used to transport siRNA into cells. Use of cell-penetration peptides can increase the transport efficiency of extracellular synthetic nucleic acids, such as siRNAs, to help increase concentration of the siRNA at the desired cellular location. This peptide is based on NickFect55 (NF55) which is a peptide that can efficiently transport plasmid DNA into cells in vivo. To improve the efficiency of nucleic acid delivery, this peptide has been developed to improve the intracellular release mechanism resulting in higher local dosage of the cargo. The pH sensitivity of the peptide allows for association to siRNA cargo, transport into cells, and dissociation of the siRNA in the appropriate cellular compartment (lysosomal or endosomal compartments).
- Function: siRNA delivery
- Tag: arahidyl (20-carbon) fatty acid chain attached to the N-terminus of the peptide
- Expression system: Synthetic
- Additional notes: There is an arahidyl (20-carbon) fatty acid chain attached to the N-terminus of the peptide. The sequence in brackets is attached to the sidechain amino group rather than the a-amino group. NF70 is a pH-activated, histidine-rich cell-penetrating peptide with low cell toxicity. It also has low membrane activity at physiological pH and forms stable nanoparticles in combination with siRNAs. This has been used to successfully knockdown genes in vivo and in vitro.
Handling
- Shipping conditions: Dry Ice
Target Details
- Target: Other
Application Details
- Application notes: There is an arahidyl (20-carbon) fatty acid chain attached to the N-terminus of the peptide. The sequence in brackets is attached to the sidechain amino group rather than the Îą-amino group. NF70 is a pH-activated, histidine-rich cell-penetrating peptide with low cell toxicity. It also has low membrane activity at physiological pH and forms stable nanoparticles in combination with siRNAs. This has been used to successfully knockdown genes in vivo and in vitro.
References
- Porosk et al. 2019. Biomater Sci. 7(10):4363-4374. PMID: 31411219.
