pETHSUL vector

Tool Details

*FOR RESEARCH USE ONLY

• Tool name: pETHSUL vector
• Tool sub type: pET
• Backbone size without insert: 5677
• Bacterial resistance: Ampicillin
• Description: Protein Purification; Protein Biochemistry
• Additional notes: The pETHSUL vector is part of a set of ligation independent cloning SUMO fusion vectors that allow for recombinant proteins to be produced with any desired sequence at the amino terminus, so long as the initial residue is not proline. This set of vectors generates affinity tagged SUMO fusion target proteins that allow the target protein to be expressed in E.coli and purified using subtractive affinity chromatography. The purified SUMO fusion is treated with recombinant SUMO protease (generated by pdtUD1 vector) to yield the mature target protein. The pETHSUL vector yields His6 tagged SUMO fusion proteins. Recombinant SUMO protease cleaves His6 SUMO from the purified target protein to yield the final mature protein. The pETHSUL vector was constructed based on the pET vector architecture. Instructions for vector expression, purification, and use are outlined in: Weeks, S. D., Drinker, M., & Loll, P. J. (2007). Ligation independent cloning vectors for expression of SUMO fusions. Protein expression and purification, 53(1), 40–50. doi:10.1016/j.pep.2006.12.006 Related product: pdtUD1 vector

Target Details

• Target: His6 tagged small ubiquitin related modfiier (SUMO) fusion target protein

Application Details

• Application notes: Protein Purification; Protein Biochemistry

References

• Weeks et al. 2007. Protein Expr Purif. 53(1):40-50. PMID: 17251035.