Cat. #156496
Anti-pS114-BRCA1 [3C10G8]
Cat. #: 156496
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 3-4 weeks
Target: pS114-BRCA1
Class: Monoclonal
Application: IF ; IP ; WB
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Jo Morris ; Ruth Densham
Institute: University of Birmingham
Tool Details
*FOR RESEARCH USE ONLY
- Name: Anti-pS114-BRCA1 [3C10G8]
- Alternate name: pS114, BRCA1
- Clone: BRCA1(pS114) antibody
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Host: Mouse
- Disease: Breast Cancer
- Application: IF ; IP ; WB
- Description: BRCA1 proteins protect stalled replication forks from degradation by nucleases, through pathways that involve RAD51. Specifically, BRCA1 in complex with BARD1, and not the canonical BRCA1-PALB2 interaction, is required for fork protection. BRCA1-BARD1 is regulated by a conformational change mediated by the phosphorylation-directed prolyl isomerase PIN1. PIN1 activity enhances BRCA1-BARD1 interaction with RAD51, thereby increasing the presence of RAD51 at stalled replication structures.
Target Details
- Target: pS114-BRCA1
- Target background: BRCA1 proteins protect stalled replication forks from degradation by nucleases, through pathways that involve RAD51. Specifically, BRCA1 in complex with BARD1, and not the canonical BRCA1PALB2 interaction, is required for fork protection. BRCA1BARD1 is regulated by a conformational change mediated by the phosphorylation-directed prolyl isomerase PIN1. PIN1 activity enhances BRCA1BARD1 interaction with RAD51, thereby increasing the presence of RAD51 at stalled replication structures.
Applications
- Application: IF ; IP ; WB
Handling
- Format: Liquid
- Concentration: 0.9-1.1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15° C to -25° C
- Shipping conditions: Shipping at 4° C
References
- Daza-Martin et al. 2019. Nature. 571(7766):521-527. PMID: 31270457.
