Cat. #154650
TCL1-870 cell line
Cat. #: 154650
Sub-type: Continuous
Unit size: 1x10^6 cells / vial
Availability: 10-12 weeks
Organism: Mouse
Tissue: Lymphatic Tissue
Disease: Cancer
Model: Cancer Model
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Dimitar Efremov
Institute: International Centre For Genetic Engineering And Biotechnology (ICGEB)
Tool Details
*FOR RESEARCH USE ONLY
- Name: TCL1-870 cell line
- Tool sub type: Continuous
- Parental cell: E-TCL1
- Organism: Mouse
- Tissue: Lymphatic Tissue
- Disease: Cancer
- Model: Cancer Model
- Conditional: No
- Description: The proliferation and survival of chronic lymphocytic leukemia (CLL) B-cells is regulated by intracellular signaling pathways which are activated by various stimuli such as antigenic stimuli propagaged through the B-cell receptor (BCR). For this reason inhibition of antigen-dependent BCR signalling can be considered a promising therapeutic approach in CLL. Some studies have shown that sustained engagement of the BCR induces a powerful antiapoptotic program in CLL cells. Transduction of the pr...
- Production details: Established from an EÄÂ??-TCL1 transgenic colony which had a B6/C3H background. Adoptive-transfer experiments with this leukemia was done in B6/C3H F1 recipients (6- to 8-week-old female mice). For adoptive transfer, 1.5 x 10^7 TCL1 leukemia cells were thawed, resuspended in 500 ÄÂ??L of phosphate-buffered saline (PBS), and injected intraperitoneally into the syngeneic recipients. Mice were followed for leukemia development and were euthanized when they developed signs and symptoms of...
Target Details
- Target: e.g. chronic lymphocytic leukemia
Applications
- Application notes: TCL1-870 murine leukaemia cells are splenic B cells which express high levels of phosphorylated Syk. They have low viability in vitro.
Handling
- Format: Frozen
- Growth medium: Must be propagated in vivo.
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
References
- The Syk inhibitor fostamatinib disodium (R788) inhibits tumor growth in the E-TCL1 transgenic mouse model of CLL by blocking antigen-dependent B-cell receptor signaling.