#154650

TCL1-870 cell line

Cat. #154650

TCL1-870 cell line

Cat. #: 154650

Sub-type: Continuous

Unit size: 1x10^6 cells / vial

Availability: 10-12 weeks

Organism: Mouse

Tissue: Lymphatic Tissue

Disease: Cancer

Model: Cancer Model

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Dimitar Efremov

Institute: International Centre For Genetic Engineering And Biotechnology (ICGEB)

Tool Details
Target Details
Applications
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: TCL1-870 cell line
  • Tool sub type: Continuous
  • Parental cell: E-TCL1
  • Organism: Mouse
  • Tissue: Lymphatic Tissue
  • Disease: Cancer
  • Model: Cancer Model
  • Conditional: No
  • Description: The proliferation and survival of chronic lymphocytic leukemia (CLL) B-cells is regulated by intracellular signaling pathways which are activated by various stimuli such as antigenic stimuli propagaged through the B-cell receptor (BCR). For this reason inhibition of antigen-dependent BCR signalling can be considered a promising therapeutic approach in CLL. Some studies have shown that sustained engagement of the BCR induces a powerful antiapoptotic program in CLL cells. Transduction of the pr...
  • Production details: Established from an EÂ?„?ž-TCL1 transgenic colony which had a B6/C3H background. Adoptive-transfer experiments with this leukemia was done in B6/C3H F1 recipients (6- to 8-week-old female mice). For adoptive transfer, 1.5 x 10^7 TCL1 leukemia cells were thawed, resuspended in 500 Â?„?žL of phosphate-buffered saline (PBS), and injected intraperitoneally into the syngeneic recipients. Mice were followed for leukemia development and were euthanized when they developed signs and symptoms of...

Target Details

  • Target: e.g. chronic lymphocytic leukemia

Applications

  • Application notes: TCL1-870 murine leukaemia cells are splenic B cells which express high levels of phosphorylated Syk. They have low viability in vitro.

Handling

  • Format: Frozen
  • Growth medium: Must be propagated in vivo.
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice

References

  • The Syk inhibitor fostamatinib disodium (R788) inhibits tumor growth in the E-TCL1 transgenic mouse model of CLL by blocking antigen-dependent B-cell receptor signaling.