ZH3D7 somatic hybrid cell line panel

Tool Details

*FOR RESEARCH USE ONLY

• Name: ZH3D7 somatic hybrid cell line panel
• Tool sub type: Continuous
• Parental cell: ZH3D7
• Organism: Human
• Tissue: Breast
• Disease: Cancer
• Model: Cancer Model
• Conditional: No
• Description: Endocrine therapy of breast cancer has been applied widely and proven to be effective. However, in many instances endocrine treatments ultimately fail due to the development of an estrogen-independent therapy resistant phenotype. To elucidate the molecular mechanism underlying this endocrine therapy failure, the laboratory of Lambert Dorssers applied random insertional mutagenesis using defective retroviruses to identify the main genes conferring estrogen independence. Out of 15 candidate B...
• Production details: Hygromycin-B-resistant variants of ZR-75-1 cells, ZH3D7, were used as recipients in the somatic-cell-fusion experiments. Donor cells were two different anti-estrogen-resistant cell lines. Approximately 6 million donor cells, which were gamma-irradiated with approx 40 Gy and 3 million recipient cells were plated in 25cm flasks in RBCS medium with estradiol. After strong adherence to the flasks in 36 to 48 hr, cells were washed 3 times in RPMI-1640 without serum and incubated with 1 ml polyethy...

Target Details

• Target: BCAR1, BCAR2

Applications

• Application notes: These cell lines are resistant to hygromycin and Geneticin and are maintained in RBCS medium with estradiol and Geneticin. Since they carry a BCAR gene, that can also proliferate slowly in medium without estradiol and supplemented with anti-estrogen.

Handling

• Format: Frozen
• Growth medium: RBCS medium containing estradiol and Geneticin (G418)
• Unit size: 1x10^6 cells / vial
• Shipping conditions: Dry ice

References

• Brinkman et al. 2000. J Natl Cancer Inst. 92(2):112-20. PMID: 10639512.
• Dorssers et al. 1997. Int J Cancer. 72(4):700-5. PMID: 9259413.