#154624

ZR-75-1 AKT2 [ZR-AKT 2 pool B] cell line

Cat. #154624

ZR-75-1 AKT2 [ZR-AKT 2 pool B] cell line

Cat. #: 154624

Sub-type: Continuous

Unit size: 1x10^6 cells / vial

Availability: 3-4 weeks

Organism: Human

Tissue: Breast

Disease: Cancer

Model: Cancer Model

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Lambert Dorssers

Institute: Erasmus University Medical Center (Erasmus MC)

Tool Details
Target Details
Applications
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: ZR-75-1 AKT2 [ZR-AKT 2 pool B] cell line
  • Alternate name: AKT Serine/Threonine Kinase 2; PKB Beta; Rac Protein Kinase Beta
  • Tool sub type: Continuous
  • Parental cell: ZR-75-1
  • Organism: Human
  • Tissue: Breast
  • Disease: Cancer
  • Model: Cancer Model
  • Description: Breast cancer is widely and effectively treated with endocrine treatment. However, in many cases the tumours will eventually progress into an estrogen-independent and therapy-resistant phenotype. Seven genes including AKT1, AKT2, BCAR1, BCAR2, BCAR3, EGFR2 and GRB7 have been shown to directly underlie estrogen independence in human breast cancer cells. This cell line is part of a panel of 16 cell lines (Cat No 154621-154635, 154642) which have been transfected with these genes, plus the pare...
  • Production details: Expression constructs with AKT2 were transfected into ZR-75-1 cells using FuGENE 6 and selected with G418. After selection for G418 resistance colonies were pooled and expanded
  • Biosafety level: 1

Target Details

  • Target: AKT2

Applications

  • Application notes: The cell line is resistant to Geneticin, which may be included in the culture medium to ensure that the expression vector is retained by the cells.

Handling

  • Format: Frozen
  • Growth medium: RPMI 1640 medium supplemented with 10% heat-inactivated bovine serum (RBCS) and 1 nM 17?-estradiol
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice
  • Storage conditions: Liquid Nitrogen
  • Mycoplasma free: Yes

References

  • van Agthoven et al. 2009. Breast Cancer Res Treat. 114(1):23-30. PMID: 18351453.