RBMXL2 conditional KO mouse

Tool Details

*FOR RESEARCH USE ONLY

• Tool name: RBMXL2 conditional KO mouse
• Alternate name: RBMX Like 2, Testis-Specific Heterogeneous Nuclear Ribonucleoprotein G-T, RNA-Binding Motif Protein, X-Linked-Like-2, HNRNPGT
• Tool sub type: Mouse
• Disease: Male infertility
• Model: Conditional KO
• Conditional: Yes
• Conditional description: Crossing with a Cre expressing mouse is required for knockdown
• Description: DNA within a gene is transcribed to make molecules of ribonucleic acid (RNA). The cell then modifies many of these RNAs in a process called splicing before using them as templates to make proteins. The RBMXL2 protein is involved in pre-mRNA processing, metabolism and transport and is only made during and after meiosis in humans and most other mammals. RBMXL2 is primarily localized to the nuclei of meiotic spermatocytes and as such is a candidate gene for autosomal male infertility
• Phenotype: Male KO mice had smaller testis devoid of sperm. No effect on female fertility
• Zygosity: Homozygous
• Strain: C57BL/6
• Production details: A targeting construct in which the Rbmxl2 open reading frame was flanked by LoxP sites was made using standard molecular biology techniques, and electroporated into ES129 cells. Positive clones were injected into blastocysts to create chimaeras and bred to yield agouti pups heterozygous for the targeted locus. The original mice containing the Neomycin gene were crossed to FlpE mice to remove the Neo gene and to generate the Rbmxl2 LoxP conditional allele. Crossing with a Cre expressing mouse is required for knockdown

Target Details

• Target: RBMXL2

References

• Ehrmann et al. 2019. Elife. 8:. PMID: 30674417