#154163

LNCaP: ARF876L cell line

Cat. #154163

LNCaP: ARF876L cell line

Cat. #: 154163

Sub-type: Continuous

Unit size: 1x10^6 cells / vial

Availability: 10-12 weeks

Organism: Human

Disease: Cancer

Model: Tumour line

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Institute: Northern Institute For Cancer Research, Newcastle University

Tool Details
Target Details
Handling

Tool Details

*FOR RESEARCH USE ONLY

  • Name: LNCaP: ARF876L cell line
  • Alternate name: AR, Dihydrotestosterone Receptor, Nuclear Receptor Subfamily 3 Group C Member 4, NR3C4, DHTR
  • Tool sub type: Continuous
  • Parental cell: LNCaP
  • Organism: Human
  • Disease: Cancer
  • Model: Tumour line
  • Conditional: No
  • Description: LNCaP cells are androgen-sensitive human prostate adenocarcinoma cells derived from the left supraclavicular lymph node metastasis from a 50-year-old Caucasian male in 1977. The androgen receptor (AR) in LNCaP cells harbours a T877A mutations which enables the anti-androgen flutamide to act as an agonist. This cell line is the most commonly used for prostate cancer research. In approximately 30% CRPC, AR mutations are detected and are likely selected for under pressure by hormonal therapies....
  • Production details: HEK 293T cells were transfected with pLenti-FLAG-ARF876L to generate viral particles. Using a multiplicity of infection of 0.3 and 0.1, LNCaP cells were transduced with virus. Stable expressing clones were selected with 10Â?„?žg/ml blasticidin. Ectopic AR expression was determined by western blot.

Target Details

  • Target: Androgen Receptor

Handling

  • Format: Frozen
  • Growth medium: RPMI-1640 + 10% FBS
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice