Cat. #154127
Leprosy T Cell Line
Cat. #: 154127
Unit size: 1x10^6 cells / vial
Availability: 3-5 days
Organism: Human
Disease: Leprosy
Model: Primary line
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Pranab K Das
Tool Details
*FOR RESEARCH USE ONLY
- Name: Leprosy T Cell Line
- Parental cell: Skin of leprosy patient
- Organism: Human
- Disease: Leprosy
- Model: Primary line
- Description: Leprosy is an ancient disease caused by gram positive, rod shaped bacilli called Mycobacterium leprae. Patients present with varied clinico-pathological disease depending on the host immune response to Mycobacterium leprae. Lepromatous (LL) patients represent with low T cell and high humoral immune response. The Th2 paradigm is thought to underlye lepromatous disease. Primary T-cells isolated from skin biopsis can be used as a research tool.
- Production details: T cell lines were generated from fresh skin biopsies. Biopsies were incubated in a 24 well plate coated with 10ÄÂ??g/well fibronectin to facilitate spontaneous migration of T cells from the biopsy into the Iscove's Modified Dulbecco Medium (IMDM) supplemented with 10% normal human serum, 1mM glutamine, 100U/ml penicillin and 100ÄÂ??g/ml streptomycin. After 5 days extravasated skin T cells were transferred to an uncoated 24 well plate and expanded by mitogenic stimulation with 0.05% PH...
Handling
- Format: Frozen
- Growth medium: Iscove's Modified Dulbecco Medium (IMDM) supplemented with 10% normal human serum, 1mM glutamine, 100U/ml penicillin and 100 ??g/ml streptomycin plus mitogenic stimulation with 0.05% PHA in the presence of irradiated allogeniec feeder cells consisting of PBMCs from two unrelated donors EBV transformed B cells and 10U/ml recombinant human IL-2 for 10 days.
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
