Cat. #154107
Vitiligo Melanocyte Cell Line, Perilesional
Cat. #: 154107
Unit size: 1x10^6 cells / vial
Availability: 3-5 days
Organism: Human
Disease: Vitiligo
Model: Primary line
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Pranab K Das
Tool Details
*FOR RESEARCH USE ONLY
- Name: Vitiligo Melanocyte Cell Line, Perilesional
- Parental cell: Peri-Lesional skin of vitiligo patient
- Organism: Human
- Disease: Vitiligo
- Model: Primary line
- Conditional: No
- Description: Vitiligo is a long-term skin condition characterised by patches of the skin losing their pigment. The patches of skin affected become white and usually have sharp margins. The exact cause of vitiligo is unknown however it is believed to be due to genetic susceptibility that is triggered by an environmental factor such that an autoimmune disease occurs. This results in the destruction of skin pigment cells. Risk factors include a family history of the condition or other autoimmune diseases, su...
- Production details: Autologous skin was taken from patients with vitiligo using a dermatome. Epidermal cell suspension was isolated from the skin sample and seeded in cell culture medium consisting of HAMs F10 supplemented with 10ÄÂ??g/ml 12-0-tetradecanoylphorbol 13-acetate (PMA), 0.1nM isobutyl-methyl-1-xanthine (IBMX), 1% Ultroser G, 2mM glutamine, 100 IU/ml penicillin and 100ÄÂ??g/ml streptomycin. Overgrowth of fibroblasts and keratinocytes was prevented by addition of geneticine 1/100 (G418).
Handling
- Format: Frozen
- Growth medium: HAMs F10 supplemented with 10 ??g/ml 12-0-tetradecanoylphorbol 13-acetate (PMA), 0.1nM isobutyl-methyl-1-xanthine (IBMX), 1% Ultroser G, 2mM glutamine, 100 IU/ml penicillin and 100??g/ml streptomycin. Overgrowth of fibroblasts and keratinocytes was prevented by addition of geneticine 1/100 (G418)
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
References
- Wankowicz-Kalinska et al. 2003. Lab Invest. 83(5):683-95. PMID: 12746478.
